What is the principle of barnase barstar gene system?
The barnase-barstar transgene based hybrid seed production technology has been commercially deployed in crops like Brassica napus (Mariani et al. 1990; 1992). The system utilizes the expression of barnase gene, encoding cytotoxic RNase protein in the tapetum cells to make one of the combiner’s male sterile.
What is transgenic male sterility?
Transgenic male sterility is controlled by foreign nuclear genes. In this system two types of transgenes, viz., barnase and barstar are used. The gene barnase causes male sterility and the gene barstar restores the fertility. Both these genes have been utilized from the bacterium Bacillus amyloliquefaciens.
Which method is used to cytoplasmic male sterility?
In cytoplasmic–genetic male sterility restoration of fertility is done using restorer lines carrying nuclear genes. The male-sterile line is maintained by crossing with a maintainer line carrying the same nuclear genome but with normal fertile cytoplasm.
What is phenotypic male sterility?
Male sterility in plants implies an inability to produce or to release functional pollen, and is the result of failure of formation or development of functional stamens, microspores or gametes.
What is male sterility write the different types of male sterility?
Extensive studies suggest that genetic male sterile lines can be broadly classified into three categories; photoperiod genetic male sterile (PGMS), thermosensitive genetic male sterile (TGMS), and photoperiod and thermosensitive genetic male sterile (PTGMS) [15, 24, 25].
Which line is male sterile line?
Such a male fertile line is known as maintainer line or ‘B’ line and male sterile line is also known as ‘A’ line.”
What are the types of male sterility?
What is male sterility and types of male sterility?
Abstract. Male sterility is characterized by nonfunctional pollen grains, while female gametes function normally. stamens, microspores or gametes.
What is CGMS system of MS?
Cytoplasmic genic male sterility (CGMS) This is a case of cytoplasmic male sterility where a nuclear gene for restoring fertility in the male sterile line is known. The fertility restorer gene, RR is dominant and is found in certain strain of the species or may be transferred from a related species.
Who certifies seeds in India?
Certification Agency
Concerned Authority. As per the provisions under Section 8 of the Seeds Act, 1966, the seed certification will be performed by the Certification Agency.
What is the main function of cytoplasm?
The cytoplasm is the gel-like fluid inside the cell. It is the medium for chemical reaction. It provides a platform upon which other organelles can operate within the cell. All of the functions for cell expansion, growth and replication are carried out in the cytoplasm of a cell.