How do you ProLong gold Antifade reagent?
Directions for Use: Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip. To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.
How Long does ProLong Gold take to cure?
24 hours
Like the original ProLong® antifade reagent, ProLong® Gold reagent will cure within 24 hours, and the sample can be saved for months after mounting.
How do you ProLong Antifade?
It comes ready-to-use (room temperatures storage and no mixing required)—just apply a drop to the sample, add a coverslip, cure, and image. ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP.
How do you ProLong gold Antifade reagent with DAPI?
Product Usage Information Following immunostaining, cover samples with ProLong® Gold Antifade Reagent with DAPI #8961 and coverslip. To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.
What does ProLong gold do?
ProLong Gold mountant contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal.
Does ProLong gold Harden?
Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents.
Does ProLong gold have glycerol?
When comparing this product to the competition’s mounting media, keep in mind that ProLong® Gold is a glycerol-based hard mount media. This means that it cures over 24 hours to a solid state so coverslips do not need to be sealed to the slide with products like nail polish or beeswax.
How do you make a mounting solution?
Mounting media can be prepared in the lab or commercially available ones can be purchased from several of the companies dealing in fluorescent probes. Mounting medium can be made with 9 parts of glycerol and 1 part PBS. The pH should be adjusted to between 8.5 and 9.0.
How do you ProLong gold?
What is vectashield?
Description. VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.
How does ProLong gold work?
Does Fluoromount G Harden?
Fluoromount-G® Anti-Fade is a non-hardening, aqueous-based compound for mounting slides used in microscopy applications in which the preservation of fluorescence intensity is critical.