What is the principle in fluorescence immunoassay?
A modern fluorescent based immunoassay uses as the detection reagent a fluorescent compound which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.
What is fluoro enzyme immunoassay?
Enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. It is used as a diagnostic tool in medicine and pathology, as well as a quality-control test across industries.
How does fluorescence polarization immunoassay work?
Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen. The principle of the assay is that a fluorescent dye (attached to an antigen or an antibody fragment) can be excited by plane-polarized light at the appropriate wavelength.
What is FIA assay?
Fluorescence immunoassay (FIA) is a simple, rapid, and sensitive technique that is used to measure many compounds including drugs, hormones, and proteins. This method has been widely applied in the in vitro diagnostics (IVD) industry.
What are immunoassays used for?
Immunoassays are biochemical tests used to detect the presence or concentration of a specific chemical, such as a toxin or hormone, in a solution using antibody-antigen reactions.
What is chemiluminescent immunoassay?
Chemiluminescence immunoassay (CLIA) is an assay that combine chemiluminescence technique with immunochemical reactions. Similar with other labeled immunoassays (RIA, FIA, ELISA), CLIA utilize chemical probes which could generate light emission through chemical reaction to label the antibody.
What is fluorescence polarization technique?
The technique of fluorescence polarization (FP) is based on the observation that when a fluorescently labeled molecule is excited by polarized light, it emits light with a degree of polarization that is inversely proportional to the rate of molecular rotation.
What is fluorescence polarization spectroscopy?
Fluorescence polarization spectroscopy is one such method, which studies the relationship between the polarization of light that is used for excitation and light that is subsequently detected from fluorescence.
What is time-resolved fluoroimmunoassay screening?
In time-resolved fluoroimmunoassay (FIA), the unusually long fluorescence decay time of the lanthanide element Europium (Eu+3) is used to discriminate the fluorescence decay characteristics of Europium-conjugated antibodies (Europium is first chelated with ethylenediaminetetraacetic acid [EDTA]) from the fluorescence …
What is a fluorescence immunoassay used for?
Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. Homogeneous fluorescence im …
What are the different types of immunofluorescent assay?
• Briefly compare direct immunofluorescent, inhibition immunofluorescent, and indirect immunofluorescent assays. • Describe the advantages, disadvantages, and application of Q dots, SQUID technology, luminescent oxygen-channeling immunoassay, fluorescent in situ hybridization, signal amplification technology, and magnetic labeling technology.
What is huorescence immunoassay used for?
Fluorescence Immunoassay JOCELYN M. HICKS, PhD* Huorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, po- tentially, bacteria.
What are the different types of immunoassays?
Immunoassays can be divided into two types, heterogeneous and homogeneous immunoassays. Heterogeneous immunoassays involve a solid phase (microwell, bead) and require washing steps to remove unbound antigens or antibodies.