How many base pairs is pUC19?
2686 base pairs
The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
What does pUC19 mean?
plasmid cloning
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
Does pUC19 have a T7 promoter?
Expresses the “Top” transcript of the Split-Broccoli system under the control of the T7 promoter. …
How is pUC19 made?
The plasmid will be made by removing, through restriction digests, a 1.7 kb fragment of pBR322, was prepared and purified by gel electrophoresis and extraction. This fragment was isolated and ligated into a pUC19 that was linearized by a restriction digest, using an enzyme with a single recognition site on the plasmid.
What is the host of plasmid pUC19?
Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
Why is pUC19 a good cloning vector?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
Is pUC19 expression vector?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
Can pUC19 be used as expression vector?
Cloning vectors can be used for expression. I’ve done it myself with both pUC19 and pBluescript (using the lac promoter in both cases; haven’t tried the T7 promoter in pBluescript). 4- Most cloning vectors lack terminators around the MCS.
What is pUC19 vector used for?
pUC19 Vector. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
How many base pairs does pUC19 have?
It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the most widely used vector molecules as the recombinants, or the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media.
What is the origin of replication of pUC19?
The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number.
Which media should be used for pUC19 plasmid cloning?
Therefore, the media used should contain ampicillin, IPTG, and X-gal . Due to its extensive use as a cloning vector in research and industry, pUC19 is frequently used in research as a model plasmid.