How is nalidixic acid stock solution prepared?

How is nalidixic acid stock solution prepared?

Solvent used for preparation of stock solution of nalidixic acid: 1/2 volume of water, then add 1mol/L NaoH dropwise to dissolve. Thank you so much for your answer , will surely try it. The acid is not soluble in water (0.1 mg/mL) but the sodium salt is (100 mg/mL).

How do you make a stock solution of antibiotics?

Preparation of 80ml stock solution

1. Tetracycline is kept in the 4C fridge in 68-564D.
2. Weigh 400mg of tetracyline HCL into a small weigh boat.
3. Dilute 95% Ethanol to 70% using milliQ water.
4. Add 80ml of 70% Ethanol to a 250ml bottle.
5. Add the tetracycline HCL to the ethanol.

How do you make Cipro stock solution?

Ciprofloxacin reagent preparation

1. Dissolve 25 mg of ciprofloxacin powder into 25 mL of DEPC water to make 1 mg/mL ciprofloxacin stock solution.
2. Dilute the stock solution by adding 80 µL of that solution to 8 mL of DEPC water with 0.1% Tween 20, resulting in a 10 µg/mL concentration.

Is nalidixic acid gram positive or negative?

It possesses Gram-positive activity and lacks cross-resistance to every clinically significant resistance mechanism tested. It is active against VRE, MRSA, penicillin-resistant pneumococci, cephalosporin-resistant bacteria, multidrug-resistant Mycobacterium tuberculosis and M.

How do you make nalidixic acid?

Nalidixic acid (30 mL) 360 mg NaOH. 30 mL ddH2O. Filter sterilize.

Why are stock solutions prepared?

Stock solutions are used to save preparation time, conserve materials, reduce storage space, and improve the accuracy with which working lower concentration solutions are prepared.

Can Cipro be dissolved in water?

Ciprofloxacin shows a poor solubility in water and in the two studied alcohols, and the solubility in acetone is usually 1 order of magnitude larger than in the other solvents.

How do you make 100mm stock?

416.6 mg in 10 ml will give you 100 mM solution. This can be aliquoted into multiple vials and kept frozen. When needed, thaw one vial and then use this stock soluition to make further dilutions.

What is nalidixic acid target?

Nalidixic acid concentrates in the renal tubules and bladder and exerts its local antibacterial actions by interference of DNA gyrase activity, thereby inhibiting DNA synthesis during bacterial replication in a dose-dependent manner.

What is nalidixic acid used to treat?

NegGram (nalidixic acid, USP) is indicated for the treatment of urinary tract infections caused by susceptible gram-negative microorganisms, including the majority of E. Coli, Enterobacter species, Klebsiella species, and Proteus species.

What is nanalidixic acid?

Nalidixic acid (NA) is one of the oldest quinolone antibiotic used for the treatment of urinary tract infection.

How do you make nalidixic acid with ethyl iodide?

Hydrolyzing the resulting product with a base gives the corresponding acid (33.2.3). Alkylating this with ethyl iodide in the presence of potassium hydroxide gives nalidixic acid [60–64 ]. Nalidixic acid has a bactericidal or bacteriostaic effect depending on the sensitivity of the microorganism and the concentration.

What is nalidixic acid sodium salt used for?

Nalidixic acid sodium salt is an inhibitor of bacterial DNA polymerase (DNA gyrase) and avian myeloblastoma virus reverse transcriptase. Inhibits nucleic acid and protein synthesis in Saccharomyces cerevisiae.

What is 333374-05-8 nalidixic acid?

3374-05-8 (hydrochloride salt, anhydrous) Nalidixic acid is a cream-colored powder. (NTP, 1992) National Toxicology Program, Institute of Environmental Health Sciences, National Institutes of Health (NTP). 1992. National Toxicology Program Chemical Repository Database.