Who can use the Bio-Rad reliance SARS-Cov-2 RT-PCR assay kit?

Who can use the Bio-Rad reliance SARS-Cov-2 RT-PCR assay kit?

The Bio-Rad Reliance SARS-CoV-2 RT-PCR Assay Kit is intended for use by qualified clinical laboratory personnel specifically trained and instructed in RT-PCR techniques and in vitro diagnostic procedures. The Bio-Rad Reliance SARS -CoV-2 RT -PCR Assay Kit is only for use under the Food and Drug Administration ’s

What are the guidelines for designing primers for a qPCR assay?

Designing Primers for a qPCR Assay When designing primers, follow these guidelines: Design primers that have a GC content of 50–60% Strive for a T m between 50 and 65°C.

How do I select the right fluorophores for multiplex qPCR assays?

Multiplex qPCR assays require multiple reporters — one to follow each amplification reaction. To distinguish each reaction, choose reporter fluorophores with minimally overlapping emission spectra. The selected fluorophores must also be compatible with the excitation and emission filters of your real-time PCR detection system.

How do I contact Bio-Rad technical support?

The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Time. Phone: 1-800-424-6723, option 2 Email: Support@Bio -Rad.com (U.S./Canada only) For technical assistance outside the U.S. and Canada, contac t your local technical support office or click the Contact us link at bio -rad.com.

Where can I get help with PCR assay design?

A number of free online resources are available to help you with PCR assay design (see Free Internet Resources for Primer Design ). Commercially available programs such as Beacon Designer software can perform both primer design and target sequence selection.

What are the general considerations when designing primers for a PCR assay?

See Real-Time PCR: General Considerations (Bio-Rad bulletin 2593) for more details When designing primers for a PCR assay, follow these guidelines: Strive for a T m between 50 and 65°C. One way to calculate T m values is by using the nearest-neighbor method (use this online T m calculator)