What does Amplex Red measure?

What does Amplex Red measure?

Reagents such as Amplex® Red have been developed for detecting hydrogen peroxide (H2O2) and are used to measure the release of H2O2 from biological samples such as mammalian leukocytes undergoing the oxidative burst.

What is Amplex Red reagent?

Additional Information. The Amplex Red reagent, a highly sensitive and stable probe for H2O2, is our best fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stoichiometry with H2O2 to produce highly fluorescent resorufin.

What is red hydrogen peroxide?

The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H2O2 released from biological samples, including cells, or generated in enzyme-coupled reactions. This reaction has been used to detect as little as 10 picomoles of H2O2 in a 100 µL volume or 1 x 10–5 U/mL of HRP.

Is Amplex Red Cell permeable?

The cells appeared fluorescent against a fluorescent background, but it should be noted that Amplex Red is cell-impermeable and is designed as an extracellular assay.

What is h2o2 assay?

The Hydrogen Peroxide Assay is for the quantitative determination of hydrogen peroxide in colorless tissue culture media and buffers. For research use only. DESCRIPTION. Hydrogen Peroxide (H202) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

Why did my skin turn white when I use hydrogen peroxide?

When you dab hydrogen peroxide on a cut, that white, fizzling foam is actually a sign that that the solution is killing bacteria as well as healthy cells.

Can peroxide burn your skin?

If you were to use a higher concentration of hydrogen peroxide on your skin, burning and blistering could occur. Even low concentrations, like 3% medical-grade options, can cause skin irritation. Swallowing hydrogen peroxide causes oxygen bubbles to form in the stomach.

How is resazurin reduced?

Resazurin is reduced to resorufin by aerobic respiration of metabolically active cells, and it can be used as an indicator of cell viability. The irreversible reaction of resazurin to resorufin is proportional to aerobic respiration.

Why does TMB turn blue?

TMB turns blue in presence of H2O2 and Peroxidase. Sometimes metals also act as peroxidase mimic. Just avoid any peroxide and peroxidase in proximity. You should consider preparing a fresh stock.

What does 20 volume H2O2 solution mean?

20 volumes of H2O2 means 20 litres of oxygen is obtained at STP by the decomposition of 1 litre H2O2 solution 2H2O2→2H2O+O2 2 mole 1 mole 68 g 22.4 litre at STP 22.4 litres oxygen is obtained from 68 g H2O2 ∴ 20 litres oxygen is obtained from = 6822.4×20=60.7gH2O2 ∴ 1000 ml of H2O2 solution contains 60.7 g H2O2 100 ml …