What is Diff Quick stain used for?

What is Diff Quick stain used for?

Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates.

How do you remove Diff-Quik stains?

1. Air-dry the smear.
2. Fix in “Diff Quick” Fixative (or methanol) for 30 secs/drain.
3. Stain with “Diff Quick” solution II for 30 secs/drain.
4. Counterstain (optional) with “Diff Quick” solution I for 30 secs/drain.
5. Rinse in tap water to remove excess stain.
6. Rapidly dehydrate in absolute alcohol.
7. Clear and mount.

What is Leishman stain procedure?

Leishman Stain is a neutral stain for blood smears which was devised by the British surgeon W. B. Leishman (1865–1926). It consists of a mixture of eosin (an acidic stain), and Methylene blue (a basic stain) in Methyl alcohol and is usually diluted and buffered during the staining procedure.

What is simple staining?

Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background.

What is Diff Quik stain made of?

The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.

What is the difference between Romanowski stain and Diff Quick stain?

“Diff-Quick” is a proprietory brand of a Romanowski stain. The Romanowski group of stains are defined as being the black precipitate formed from the addition of aqueous solutions of methylene blue and eosin, dissolved in methanol.

How do you use dipdip stain?

Dip slide or tape-strip five times, for one second each, into Stain 1. Allow excess to drain after each dip. Dip slide or tape-strip five times, for one second each, into Stain 2. Allow excess to drain after each dip.

How do you stain with thiazine dye?

Stain solution 2 (Thiazine dye in phosphate buffer) – blue colour Dip slide or tape-strip five times, for one second each, into Fixative. Allow excess to drain after each dip. Dip slide or tape-strip five times, for one second each, into Stain 1. Allow excess to drain after each dip.