What is an EF promoter?
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.
What is RSV promoter?
Aside from the CMV promoter, the Rous sarcoma virus long terminal repeat (RSV) promoter, which also directs high-level expression in a broad range of tissues and cells, is frequently used in adeno- virus transducing vectors.
What is hSyn promoter?
This biomodule encodes for Human synapsin 1 gene promoter (hSyn). hSyn confers highly neuron-specific long-term transgene expression in the adult rat brain. The AAV collection was developed by Sirion Biotech AG and is made available for research purposes only.
Is ef1a a strong promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
What is AAV Dio?
This AAV expresses DIO-EYFP driven by an ubiquitous EF1a promoter. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).
What is CBh promoter?
The CBA promoter displayed mostly ubiquitous and high neural expression, but substantially lower expression in motor neurons (MNs). We report the creation of a novel hybrid form of the CBA promoter (CBh) that provides robust long-term expression in all cells observed with CMV or CBA, including MNs.
What is CaMKII promoter?
About CaMKII Promoter: The CaMKII’s promoter is a neuron-specific promoter. Its expression restricted to excitatory neurons in the neocortex and hippocampus, also pyramidal neurons. CaMKII promoter were used for transgene expression to obtain preferential gene expression in CNS for the excitatory neuron populations.
Is PGK a constitutive promoter?
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG for mammalian systems, and COPIA and ACT5C for Drosophila systems).
Is Dio same as FLEx?
Sometimes also referred to as DiO (Double-floxed inverse Orientation), FLEx systems are generally considered “Cre-On” meaning that your gene of interest starts in the inverse/antisense “off” position and is flipped to the sense (on) orientation in the presence of Cre.
What does DIO mean in virus?
For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO-AAV (doublefloxed inverse orf) AAV.
What is ef1alpha promoter?
Why EF1alpha promoter is so special? Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.
How important is the EF-1 alpha promoter for the generation of stable cells?
In vivo the tumors carrying the EF-1 alpha promoter were homogeneously GFP-positive, whereas the CMV promoter gave rise to a scattered pattern of GFP expression. Conclusion: This study underlines the importance of the promoter for the generation of stable cell lines.
What is the difference between the EF-1 Alpha and CMV promoters?
In vivo the tumors carrying the EF-1 alpha promoter were homogeneously GFP-positive, whereas the CMV promoter gave rise to a scattered pattern of GFP expression.
Does the EF-1 alpha promoter affect GFP expression in HT-29 clones?
Results: Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to 60% of the cells were GFP-positive with expression levels varying widely between cells.