What is luciferase reporter assay?
A luciferase reporter assay is a test that investigates whether a protein can activate or repress the expression of a target gene using luciferase as a reporter protein (Carter & Shieh, 2015). This bioluminescence directly corresponds with the effect of the protein on expression of the target gene.
How do you write a reporter gene?
To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. This method is an example of using cis-acting elements where the two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule.
What is a reporter mouse?
A reporter Knockin mouse defines an animal model in which fluorescent, bioluminescent proteins or biochemical tags are inserted into the genome. The reporter can replace a gene, be fused to a protein or inserted into the 3′ UTR.
What is a reporter tag?
The use of epitope tags in recombinant DNA techniques allows the detection of proteins where specific antibodies are not available. Reporter genes (also known as protein expression markers or small reporter proteins) are used in gene expression studies and provide an indication of the transcriptional activity in cells.
What is dual luciferase assay used for?
The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly.
What is NanoLuc?
NanoLuc™ (Nluc) is a 19.1 kDa, ATP- independent luciferase that utilizes a novel coelenterazine analog (furimazine) to produce high intensity, glow-type luminescence.
What is the difference between Firefly and Renilla luciferase?
What is the Difference Between Firefly and Renilla Luciferase? Firefly luciferase assays uses luciferin in the presence of oxygen, ATP and magnesium to produce light (Green/Yellow, 550-70 nM), while Renilla luciferase assays (SCT153) requires only coelenterazine and oxygen to produce light (Blue, 480 nM).
What is the Cignal reporter assay?
Cignal Reporter Assays provided in the Cignal Reporter Assay Kit enable rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. Two reporter systems are available: dual-luciferase format and GFP format.
What is the Cignal finder array?
The Cignal Finder Arrays consist of 10 or 45 dual-luciferase reporter assays, and are designed for use in one of four research areas. The targeted research areas are cancer, immunology, development, and toxicology. In this era of post-genomics life science research, many labs are investigating how diverse signal transduction pathways function
What is a reporter assay kit used for?
Cignal Reporter Assay Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.