What is a dual reporter assay?
The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample.
What are reporter assays used for?
Reporter gene assays are invaluable for studying regulation of gene expression, both by cis-acting factors (gene regulatory elements) and trans-acting factors (transcription factors or exogenous regulators).
How do Assays work?
An assay is an analytical measurement procedure defined by a set of reagents that produces a detectable signal for quantifying a biological process. The quality of an assay is defined by the robustness and reproducibility of the signal in the absence of a test compound.
What is a reporter line?
Reporter-labeled cell lines, also simply called reporter cell lines, offer different applications compared to a normal cell line. They can be used to visualize and track the expression of proteins, transcription factors, or other molecules as well as potentially track where they are within whole cells in real-time.
How is luciferase expression detected?
To measure luciferase activity of the cell lysates, you will need a multiwell plate or a tube containing cell lysates and a luminometer. This apparatus detects the photon emission produced from the luciferase reaction and the unit of measurement comes out as relative light units (RLU).
What are the different assays?
Secretion assays. Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity assays, and Annexin V staining. Chemosensitivity assay measures the number of tumor cells that are killed by a cancer drug. Tetramer assay detect the presence of antigen specific T-cells.
What is a dual-reporter assay?
Dual-reporter assays enable researchers to obtain additional information from complex systems with minimal effort. The Dual-Luciferase®Reporter (DLR™) Assay and Dual-Glo™ Assay enable the sequential measurement of both firefly and Renillaluciferases from one sample.
What are genetic reporter assays?
Luciferase-based genetic reporter assays provide sensitive methods for assaying gene expression, enabling the accurate quantification of small changes in transcription resulting from subtle changes in biology. We have developed many simple add-mix genetic reporter assays that can be used for a variety
How is a reporter gene cloned?
Typically, a reporter gene is cloned with a DNA sequence of interest into an expression vector that is then transferred into cells. Following transfer, the cells are assayed for the presence of the reporter by directly measuring the reporter protein itself or the enzymatic activity of the reporter protein.