What is DMEM cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What are the components of DMEM?
11965 – DMEM, high glucose
| Components | Molecular Weight | Concentration (mg/L) |
|---|---|---|
| Amino Acids | ||
| Potassium Chloride (KCl) | 75.0 | 400.0 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 3700.0 |
| Sodium Chloride (NaCl) | 58.0 | 6400.0 |
How much does DMEM cost?
Refine
| Product | Product Name | Price |
|---|---|---|
| SH30022.01 | DMEM/High glucose with L-glutamine; without sodium pyruvate – 500 mL | 22.25 USD |
| SH30022.LS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 1000 mL | 192.19 USD |
| SH30022.02 | DMEM/High glucose with L-glutamine; without sodium pyruvate – 1000 mL | 34.94 USD |
What does DMEM stand for?
DMEM (Dulbecco’s Modified Eagle Medium) is the most broadly suitable medium for many adherent cell phenotypes among defined media for cell and tissue culture.
Is DMEM a basal media?
Gibco Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
How do you make DMEM media for cell culture?
DMEM (11-055-1) – 1 liter
- Prepare 900ml of distilled water in clean glass beaker.
- Add the 1-liter powder to the water and stir gently.
- Add 3.7 gram Sodium Bicarbonate (or 49.4 ml of 7.5% Sodium Bicarbonate Solution).
- Adjust pH to 0.2-0.3 units below the required pH using 1N HCl or 1N NaOH.
Why is DMEM red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).