What is FITC filter?
It is optimised for fluorescein isothiocyanate (FITC) and other fluorophores such as rsGFP, BODIPY, 5-5-FAM, Fluo-4 and Alexa Fluor 490. The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers.
How do fluorescence filters work?
Most fluorescence instruments, including fluorescence microscopes, are based on optical filters. The emitter is typically a bandpass filter that passes only the wavelengths emitted by the fluorophore and blocks all undesired light outside this band – especially the excitation light.
What does FITC stain?
FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides. Tissue sections and cell monolayers may be stained in various ways with FITC.
How does a dichroic filter work?
Dichroic filters use the principle of thin-film interference, and produce colors in the same way as oil films on water. When light strikes an oil film at an angle, some of the light is reflected from the top surface of the oil, and some is reflected from the bottom surface where it is in contact with the water.
What is microscope filters?
Microscopy filters are used to filter out specific wavelengths of light thereby increasing contrast, blocking ambient light, removing IR or UV radiation. Filters are generally fitted over the illuminating device below the iris diaphragm. The distance between a crest and a trough is called the wavelength of light.
What does FITC bind?
Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
What color is FITC?
green
FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. The color of the compound is yellow while the emitted light is green.
What is an excitation and emission filter?
As you can see, the excitation filter transmits light spanning the absorbtion spectrum, and the emission filter transmits a band of wavelengths spanning the peak emission of the fluorochrome. The dichroic attenuates the exitation light and transmits only that from the emission.
What does FITC stand for?
The Invitrogen Molecular Probes Alexa Fluor 488 dye —with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.
What are techspec® fluorescence filter sets?
Our TECHSPEC® Fluorescence Filter Sets are designed to provide brilliant images when integrated into a fluorescence microscope. The high transmission of the bandpass filters guarantees the brightest possible images, while the deep blocking ensures no unwanted light gets to the detector.
What is the difference between exciter filter and barrier filter?
Exciter filters permit only selected wavelengths from the illuminator to pass through on the way toward the specimen. Barrier filters are filters which are designed to suppress or block (absorb) the excitation wavelengths and permit only selected emission wavelengths to pass toward the eye or other detector.
What is the transmission curve of an exciter filter?
Today, most exciter filters are of the interference type. The transmission curve of a KP or SP filter shows a steep drop at the right-hand side of the curve, as illustrated in Figure 2 (a). If the exciter filter is labeled with the letter B or BP, it is a band pass filter (Figure 3).