What is a FAM fluorophore?
FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5′ or 3′ end of oligos.
What makes a compound fluorescent?
Generally molecules that fluoresce are conjugated systems. Fluorescence occurs when an atom or molecules relaxes through vibrational relaxation to its ground state after being electrically excited. The specific frequencies of excitation and emission are dependent on the molecule or atom.
What is the difference between bioluminescence and fluorescence?
Bioluminescence is visible light generated by a living organism through a chemical reaction. Fluorescence is another process that can cause things to emit light. Things that fluoresce absorb light of shorter wavelength and re-emit it as longer-wavelength light.
What is the difference between Fluorochrome and fluorophore?
As nouns the difference between fluorochrome and fluorophore is that fluorochrome is any of various fluorescent dyes used to stain biological material before microscopic examination while fluorophore is (biochemistry) a molecule or functional group which is capable of fluorescence.
What is the difference between fluorophore and chromophore?
A fluorophore is a fluorescent chemical compound that can re-emit light upon excitations that occur due to a light source. Chromophore is a part of a molecule that is responsible for the color of that molecule. This is the main difference between fluorophore and chromophore.
What is Rox in qPCR?
ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.
Is Tamra a quencher?
TAMRA dye is an effective quencher for fluorophores with emission maxima less than 560 nm. Dyes with longer wavelength emissions will not be effectively quenched by TAMRA. In addition, TAMRA has its own fluorescence which complicates data analysis due to crosstalk between the channels.
What are fluorescent substances?
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after.
Is zinc sulphide a fluorescent material?
Zinc sulfide is one of the most common phosphorescent materials. Phosphorescence is a special type of photoluminescence. Unlike fluorescent substance, a phosphorescent material emits absorbed energy at once. Interesting article.