How does a luciferase assay work?
The luciferase assay is useful to study whether a protein of interest regulates a particular gene at the transcription level. When this protein activates transcription, the cell will produce luciferase enzyme. After the addition of a lysis buffer and a substrate, a luminometer quantifies the luciferase activity.
What is the purpose of luciferase?
The power of luciferase has been harnessed by scientists to devise reactions whose light output is used to monitor biological processes including gene expression, biomolecular binding, and cell viability.
What wavelength is luciferase assay?
Therefore, although bioluminescence emitted from deep within an experimental animal can be detected with a sensitive detection system, luciferases that emit light at wavelengths >600 nm have the greatest sensitivity for in vivo imaging applications (2).
What is luciferase complementation assay?
Luciferase complementation assays (LCAs) detect protein–protein interactions within living cells using bioluminescence. In the assays, complementary DNA (cDNA) of luciferase is first split into the N- and C- terminal fragments and then fused to cDNAs of a protein pair of interest.
What is dual luciferase assay?
The dual luciferase assay is performed by sequentially measuring the firefly and Renilla luciferase activities of the same sample, with the results expressed as the ratio of firefly to Renilla luciferase activity (Fluc/Rluc).
What are the products of luciferase?
“Luciferase” has 3 results in Products.
- NanoLuc® (Nluc) Luciferase Antibody. MAB10026. 1 Citation. 1 Image. WB.
- NanoLuc® (Nluc) Luciferase Antibody. MAB100261. 1 Review. 1 Image. WB.
- Human CHIP Ubiquitin Ligase Kit – Glow-Fold Substrate. K-280. 2 Citations.
What is a luciferase reporter gene?
A commonly used reporter gene is the luciferase gene from the firefly Photinus pyralis. This gene encodes a 61-kDa enzyme that oxidizes D-luciferin in the presence of ATP, oxygen, and Mg(++), yielding a fluorescent product that can be quantified by measuring the released light.
What is the purpose of a dual luciferase assay?
The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly.