What is Hep3B?
In addition, HepG2 is hepatitis B virus negative and non-tumorigenic, but Hep3B is hepatitis B virus positive and tumorigenic (Knowles et al. 1980; Knasmuller et al. 1998).
What are HepG2 cells?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.
What are huh7 cells?
Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers.
What is cell line hepatocellular carcinoma?
Human colon carcinoma cell line, Caco-2, is used to represent an intestinal cell, and liver hepatocellular carcinoma cell line, HepG2, is used to represent the liver cell. Different cells can be used, for example, primary cells or stem cells.
Why are HepG2 cells used?
Hep G2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics, the detection of environmental and dietary cytotoxic and genotoxic (and thus cytoprotective, anti-genotoxic, and cogenotoxic) agents, understanding hepatocarcinogenesis, and for drug targeting …
What is HEK 293 used in?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
How do you maintain a Huh7 cell?
Subculture Protocol for HuH-7 To subculture: Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.
How do you maintain a huh7 cell?
Are HepG2 cells hepatocytes?
Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 will respond to stimulation with human growth hormone. Hep G2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B.
Are Jurkat CD4 or CD8?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.