How do you dispose of quick dip stains?
Collect liquid in an appropriate container or absorb with an inert material (e.g. vermiculite, dry sand, earth), and place in a suitable container for reclamation or disposal. Do not use combustible materials, such as sawdust. Do not flush to sewer.
How do you Diffquik?
Method
- Allow smears to dry.
- Dip slide or tape-strip five times, for one second each, into Fixative.
- Dip slide or tape-strip five times, for one second each, into Stain 1.
- Dip slide or tape-strip five times, for one second each, into Stain 2.
- Rinse slide or tape-strip in distilled water or Weise’s buffer, pH 7.2.
What stains Thiazine?
Chemistry and principle: It is an acidophilic metachromatic dye, which belongs to thiazine group. Therefore, it has a high affinity for acidic compounds; stains the cells / tissues blue with greater nucleic acid content.
How do you Diff-Quik?
How is a differential stain different from a simple stain?
A simple stain depends on the fact that bacteria differ chemically from their surroundings and thus can be stained with their environments. A differential stain can distinguish betweeen 2 types of bacteria because bacteria differ from one another chemically & physically to react differently to a given staining procedure.
What is quick diff?
Diff-Quik is a commercial Romanowsky stain variant, commonly used in cytological staining to rapidly stain and differentiate a variety of smears, commonly blood and non-gynecological smears, including those of fine needle aspirates.
Is an acid fast stain a differential stain?
Acid Fast Stain. Purpose: The acid-fast stain is a differential stain which distinguishes organisms with waxy cell walls that can resist decolorization with acid alcohol. How it works: Acid-fast bacteria have a waxy substance called mycolic acid in their cell walls which makes them impermeable to many staining procedures, including the Gram stain.
What is C diff stain?
Clostridium Difficile (Definition) C. Diff, it is a spore forming bacillus that when the micro environment of the colon is modified by antimicrobials, it can germinate and elaborate a toxin causing pseudo membranous colitis, a potentially lethal disease.