What is DEAE ion exchange chromatography?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations).
What is a CM column?
CM Ceramic HyperD® is a weak cation exchanger for biomolecule purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization.
Is DEAE cellulose an anion or cation exchanger?
weak anion exchanger
DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger. The Sepharose types are particularly useful for the separation of high molecular weight proteins.
How do you make a cellulose column Deae?
The DEAE cellulose is prepared by suspending 10 g of DEAE cellulose (DE 52 ion-exchange cellulose according to Whatman) in 200 mL of 0.05 N HCl, which is neutralized with 400 μE of 10 N NaOH, from which the supernatant is decanted. The cellulose is then washed three or four times with a fivefold volume of SM solution.
Is CM-cellulose an anion exchanger?
Two weak exchangers can be used for protein separation are carboxymethyl- cellulose (CM-cellulose) and diethylaminoethyl-cellulose (DEAE-cellulose). It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger.
How do you make Deae resin?
Suspend the resin in 2 CV of 0.1 M NaOH containing 0.5 M NaCl for 10 min. 1 and pour the slurry into a Buchner funnel (volume = 3 CV) while applying GENTLE suction, and allowing a flow of 1 V buffer in 5 minutes. Continue pouring in slurry until all the resin is added to the funnel.
What is carboxymethyl cellulose used for?
Carboxymethyl cellulose. Carboxymethyl cellulose ( CMC) or cellulose gum is a cellulose derivative with carboxymethyl groups (-CH 2 -COOH) bound to some of the hydroxyl groups of the glucopyranose monomers that make up the cellulose backbone. It is often used as its sodium salt, sodium carboxymethyl cellulose.
Is carboxymethylcellulose soluble in water?
Carboxymethylcellulose (CMC) is an anionic, water-soluble cellulose derivative. Solubility of CMC depends on the DP as well as the degree of substitution and the uniformity of the substitution distribution. Water solubility of CMC would increase with decreased DP and increased carboxymethyl substitution and substitution uniformity.
Is Cm-Cellulose a cation or anion exchanger?
At neutral pH the carboxymethyl group is ionized as -CH 2OCH 2COO¯ so that CM- cellulose is negatively charged, so it is a weak cation exchanger. DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger.
What is the reaction between cellulose and carboxylic acid?
The polar (organic acid) carboxyl groups render the cellulose soluble and chemically reactive. Following the initial reaction, the resultant mixture produces about 60% CMC plus 40% salts ( sodium chloride and sodium glycolate ).