Are Cre mice transgenic?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
What is a Cre mouse line?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
How is Cre mouse created?
Cre-loxP system is a widely used powerful technology for mammalian gene editing. Conditional knockout mice are then generated by breeding the Cre-driver strain with a floxed mouse strain (Figure 1B). The specificity and timing of recombination are controlled by used promoter and/or enhancer.
What are the applications of transgenic mice?
Transgenic mice are extensively used as animal models for understanding human diseases and for the production of therapeutic agents. Adequate care, however, must be exercised before extrapolating data of transgenic mice to humans.
How does the Cre system work?
The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.
What is Cre used for?
Cre recombinase is a widely used tool in the field of molecular biology. The enzyme’s unique and specific recombination system is exploited to manipulate genes and chromosomes in a huge range of research, such as gene knock out or knock in studies….
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Why mice are used as transgenic animals?
Such work laid the basis for the creation of transgenic mice genetically modified to inherit particular forms of cancer. These mice were generated as a laboratory tool to better understand the onset and progression of cancer. The advantage of such mice is that they provide a model which closely mimics the human body.
What is a transgenic mice model?
Transgenic mice are mouse models that have had their genomes altered for the purpose of studying gene functions. At Charles River, we help hundreds of global customers by delivering study-ready, transgenic mice to meet their research needs.
What is a transgenic mouse model?
What is a Cre driver?
Cre-driver lines: A special type of transgenic mouse As shown in Figure 2 below, different types of neurons, whether inhibitory, excitatory or other have specific proteins and therefore genes that would allow for the specific expression of the exogenous Cre-recombinase.
Is CRE endogenous?
What is CRE activity?
Cre recombinase-mediated DNA recombination is an established method for conditional control of gene expression in animal models. Regulation of its activity has been accomplished to impart spatial and/or temporal control over recombination of the target gene.
How does Cre recombinase work?
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.
What is Cre mice?
Cre Mice. The use of Cre recombinase and loxP sites in genetically modified mouse models allows for tissue specific control over genetic modifications, enhancing our understanding of gene functions. Hundreds of different Cre mice are already available, with different patterns of Cre expression allowing conditional knockout in different tissues.
What is a knockout mouse?
Knockout Mice Fact Sheet A knockout mouse is a laboratory mouse in which researchers have inactivated, or “knocked out,” an existing gene by replacing it or disrupting it with an artificial piece of DNA. What are knockout mice used for? Knocking out the activity of a gene provides valuable clues about what that gene normally does.