How does the concentration of catalase affect hydrogen peroxide?
Catalase is a very common enzyme that is present in almost all organisms that are exposed to oxygen. To prevent such damage, the catalase enzyme helps getting rid of these compounds by breaking up hydrogen peroxide (H2O2) into harmless water and oxygen.
How does the concentration of catalase affect the rate of reaction of catalase?
As the concentration of the substrate was increased, the rate of the reaction also increased for reasons similar to the concentration of the enzyme. As the concentration of substrate increased there were more substrate molecules to bond with the active site of the catalase enzyme.
How does concentration affect hydrogen peroxide?
This is because as the concentration decreased, the number of molecules of hydrogen peroxide also decreased. This decreased the number of particles that could react with each other, and so the number of collisions that reached the activation energy also decreased.
How would using a lower concentration of hydrogen peroxide affect the rate of reaction?
The rate of reaction will increase as the hydrogen peroxide concentration increases. However, from a certain concentration, the rate will stay the same, even if the concentration increases, because the amount of enzyme used is fixed.
How fast does catalase work on hydrogen peroxide?
WHEN catalase is added to hydrogen peroxide, there is an initial rapid evolution of oxygen which lasts for about two minutes, depending on the peroxide concentration. After this, oxygen is given off at a steady rate which slowly decreases in the course of an hour.
Why did the catalase function level off when different hydrogen peroxide concentrations were tested?
Why did the catalase function level off when different hydrogen peroxide concentrations were tested? At some point, all the catalase in solution was catalyzing reactions, so even if the hydrogen peroxide concentration was increased, the rate of the reaction could not increase any more.
When catalase reacts with hydrogen peroxide the products produced are?
catalase, an enzyme that brings about (catalyzes) the reaction by which hydrogen peroxide is decomposed to water and oxygen.
How does mixing hydrogen peroxide and water test the reactivity of catalase?
How does mixing hydrogen peroxide and water test the reactivity of catalase? It shows that hydrogen peroxide is not the chemical causing the reaction. Increasing substrate concentration leads to an increase in the rate of reaction until the enzyme is saturated with substrate.
How can we know if catalase is working?
Add one drop of hydrogen peroxide and look for bubbles. Bubbles are a positive result for the presence of catalase. If no bubbles form, it is a negative result; this suggests that the organism does not produce catalase.
What factors affect catalase activity?
The rate at which an enzyme works is influenced by several factors including the concentration of substrate (hydrogen peroxide in the case of catalase), temperature, pH, salt concentration and the presence of inhibitors or activators.
Which concentration of catalase will produce the fastest reaction rate?
The concentration of catalase that had the fastest reaction time was the 75% catalase concentration, while the slowest reaction time was the 0% catalase concentration.