How long does Arabidopsis take to germinate?
The growth and development of Arabidopsis, including flowering time, is influenced by a number of environmental conditions in addition to the genetic background. Seeds of most lines germinate 3–5 days after planting under continuous light, 23 °C, adequate watering, and good nutrition.
How do you germinate old Arabidopsis seeds?
My advice is to incubate your seeds first 24 hours at room temperature in a closed box with 100% RH. The next day imbibe your seeds in water of about 20 -25 °C, definitely not cold water, on filter paper. Check daily for germinated seeds. It may take more time than usual.
How do you stratify Arabidopsis seeds?
This is my procedure: I start with sterilization (bleach 50%) and 3 days stratification (4°C in darkness). Then I sow the seeds (Columbia) on MS medium, containing 1% sugar, 0.7% agar and pH 5.7. After 2 weeks (when the first leaves appear), seedlings are transferred to soil (peat moss and perlite), in 2:1 proportion.
How long does it take for Arabidopsis thaliana to grow?
Arabidopsis thaliana is a powerful model for the study of growth and development processes in plants. It is a small plant that has a short generation time (∼6 weeks), and grows well under laboratory conditions, on shelves at room temperature, with limited amounts of light.
How long do Arabidopsis seeds last?
two years
* Arabidopsis seeds should be viable for two years in seed packets at room temperature. * High humidity is detrimental for viability (certain mutants may have a shorter shelf life).
How often do you water Arabidopsis?
Arabidopsis is sensitive to all stress conditions, so please water only when there is no standing water in the tray. If the plants are desired to proceed to flowering, water with 1/4 strength of Hoagland solution once every other week until they start bolting (twice in total is enough).
Do Arabidopsis seeds need light to germinate?
Germination of Arabidopsis seeds is light dependent and under phytochrome control. Previously, phytochromes A and B and at least one additional, unspecified phytochrome were shown to be involved in this process.
How often should you water Arabidopsis?
How long should seeds be cold stratified?
COLD STRATIFY SEEDS: PLANTING The time you need to keep your seeds in the refrigerator depends on the variety, but 4-5 weeks should be a sufficient amount of time for most seed varieties. Once there’s no more chance of frost in your area, take your seeds out of the fridge and spread seed on bare soil as normal.
How do you care for Arabidopsis?
The growth chambers can be set to different temperatures and lighting conditions, depending on the experiment. These chambers can only hold a maximum of four flats of plants, so they are generally not used for growing Arabidopsis for seed production.