How much does SYBR Green cost?
An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended….Description:
| final SYBR® Green concentration | 20 μl PCR assay | 50 μl PCR assay |
|---|---|---|
| 0.5 μM | 0.1 μl | 0.25 μl |
| 1.0 μM | 0.2 μl | 0.50 μl |
What is qPCR Master Mix?
A PCR master mix is a premixed concentrated solution that has all of the components for a real-time PCR reaction that are not sample-specific. A master mix usually contains a thermostable DNA polymerase, dNTPs, MgCl2, and proprietary additives in a buffer optimized for PCR.
How long is qPCR Master Mix good for?
Once Quant Kit Primers are added to the Master Mix, the Mix+Primers solution is stable for 6 weeks at 4 °C for convenience.
How much PCR should I load on gel?
A volume of 2 μl of purified PCR product should be loaded on the gel. After electrophoresis, bands should be easily visible. If bands are faint, the amount of template for sequencing can be increased.
How much is a SYBR?
Dilute SYBR Safe DNA Gel Stain concentrate 10,000-fold in TAE or TBE buffer prior to use. For most minigels, 50 mL of 1X stain is sufficient (e.g., dilute 5 µL of concentrate with 50 mL buffer). For larger gels, increase volumes proportionally, ensuring that the entire gel is fully immersed during staining.
What is SYBR Green mix?
SYBR® Green dye is a fluorescent double-stranded DNA (dsDNA)- binding dye that is used to track the progress of DNA amplification in real-time PCR experiments. One advantage of using a SYBR® Green master mix is that it is cost effective, particularly when compared to probe-based detection systems.
What is SYBR Green PCR?
SYBR® Green is a dsDNA-binding dye that intercalates nonspecifically into dsDNA, allowing measurement of the amount of PCR product. Since the increase in fluorescence is proportional to the amount of product accumulated, SYBR® Green qPCR can be used for relative DNA quantification.
How does SYBR Green work in qPCR?
SYBR Green is one of the most commonly used fluorescent dyes in qPCR. It binds to double-stranded DNA molecules by intercalating between the DNA bases. Once intercalated to DNA, SYBR Green becomes less mobile, causing its energy to be released as fluorescence.
Can you store PCR Master Mix?
In order to avoid the time-consuming process of thawing it is also possible to store all Ampliqon DNA polymerases, master mixes, buffers and PCR reagents at +4 °C for up to 6 months. RealQ Plus master mixes can be kept at + 4°C for up to 3 months.
What is the purpose of PCR master mix?
The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0.2–2kb. PCR Master Mix allows you to set up your reactions in less than a minute—just add template and primers. Optimized conditions enable amplification of as few as 2 copies of target template.