What are biotinylated oligos?

What are biotinylated oligos?

Biotinylation is the process of attaching a biotin tag to a molecule. The molecule can be a protein or an oligonucleotide. Biotinylated molecules are useful in many biological contexts, but are primarily used for capture or detection of target molecules.

Can RNA be biotinylated?

For example, biotinylation of RNA is used for one-step enrichment of RNA–protein complexes from complex mixtures by binding to immobilized streptavidin (Ruby and Abelson 1988). Biotin can also be incorporated at the 5′ end by enzymatic synthesis, but this precludes capping of the RNA (Huang et al. 2008).

What is biotinylated DNA?

In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol).

Why are primers biotinylated?

We recommend biotinylating the primer on the 5′ terminus to avoid interfering with the extension of the nacent strand. A biotinylated primer can be used under the same reaction conditions that would be used in an unmodified oligo.

What is biotinylated RNA?

Biotinylated RNAs are useful probes for the identification of RNA-binding proteins from different cells and tissues. The isolation of protein–RNA complexes is achieved by the ability of biotin to bind streptavidin with high affinity.

What is RNA pull down assay?

RNA pull-down assays selectively extract a protein–RNA complex from a sample. Typically, the RNA pull-down assay takes advantage of high affinity tags, such as biotin or azido-phosphine chemistry.

What are biotinylated antibodies?

Biotinylated antibodies are used for the detection of low-abundance proteins. The process of biotin-labeling is also frequently used as a non-radiative labeling method of proteins, and as a protein purification technique.

What is Pyrosequencing methylation?

Pyrosequencing is a technique that uses a sequencing-by-synthesis system which is designed to quantify single-nucleotide polymorphisms (SNPs). The ability to detect and quantify the methylation pattern of DNA has the potential to serve as an early detection marker and potential drug target for several diseases.

What is a biotinylated oligo?

Biotinylated Oligonucleotides. Biotin is widely used for DNA/RNA detection/isolation due to the extremely high affinity of the biotin-streptavidin interaction (association constant 10 15/M). Biotin moieties can be incorporated within an oligo on any place and in any number.

How does the biotinylated oligo(dT) probe work?

The Biotinylated Oligo (dT) Probe hybridizes with high efficiency to the 3´ poly (A) region present in most mature eukaryotic mRNA species.

Why do Biotinylated primers work with paramagnetic streptavidin beads?

We have discovered that in the use of biotinylated primers for sequencing reactions, the ability to capture large DNA Sanger fragment pools with paramagnetic streptavidin beads was directly related to the length of linker arms holding the biotin moiety.