What are CD45 1 mice?

What are CD45 1 mice?

CD45. 1 is an alloantigen of CD45, expressed by Ly5. 1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.

What is Ly5 1?

1 Mouse Details. The strain was originally developed at the Sloan Kettering Institute where it was backcrossed on a non-specified C57BL/6 strain. The congenic strain “C57BL/6-Ly5. 1” carries the allele of the SJL mouse in the Ptprc gene locus: “Ptprca” or “CD45.

Are B6 mice CD45 2?

2 B6 strain is the CD45 isoform. Journal Article: Jang et al., 2018. Cutting Edge: Check Your Mice−−A Point Mutation in the Ncr1 Locus Identified in CD45. 1 Congenic Mice with Consequences in Mouse Susceptibility to Infection.

What is a CD45 2 mice?

CD45. 2 congenic mice. CD45 itself is a protein tyrosine phosphatase receptor expressed on all hematopoietic cells except mature erythrocytes and platelets and encoded by the Ptprc gene. Wild-type C57BL/6 mice normally carry the Ptprcb (CD45. 2) allele while the other (congenic) strain carries the Ptprca (CD45.

How do you make a congenic mouse?

To generate a transgenic mouse, microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert the gene of interest into a zygote or into embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome.

Are B6 mice CD45 1 or CD45 2?

Two isoforms have been identified in mice: the common form is CD45. 2, which is expressed by the C57BL/6 (B6) strain and is encoded by the Ptprcb allele; and an additional allelic variant Ptprca which encodes the CD45. 1 isoform, was identified in the SJL mouse strain.

What is the difference between syngeneic and congenic?

is that syngeneic is (genetics) genetically identical, or sufficiently identical and immunologically compatible as to allow for transplantation while congenic is (genetics) (of different strains of an organism ) that are genetically identical except for a variation in a limited genetic region or locus.

What are recombinant strains?

A recombinant inbred strain (or recombinant inbred line) is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains.

What is congenic mouse model?

Glossary Term. Congenic Strain. MGI Glossary. Definition. An inbred strain of mouse that contains a small genetic region (ideally a single gene) from another strain, but which is otherwise identical to the original inbred strain.

What is Ly51 mouse?

Ly5.1 Mouse | Charles River Laboratories. The strain was originally developed at the Sloan Kettering Institute where it was backcrossed on a non-specified C57BL/6 strain. The congenic strain “C57BL/6-Ly5.1″ carries the allele of the SJL mouse in the Ptprc gene locus: ” Ptprca ” or “CD45.1” or “Ly5.1”.

Where did the c57bl/6-ly5 strain come from?

The strain was originally developed at the Sloan Kettering Institute where it was backcrossed on a non-specified C57BL/6 strain. The congenic strain “C57BL/6-Ly5.1″ carries the allele of the SJL mouse in the Ptprc gene locus: ” Ptprca ” or “CD45.1” or “Ly5.1”.

What is ncnci b6-ly5?

NCI B6-Ly5.1/Cr Mouse | Charles River Laboratories. The strain was originally developed at the Sloan Kettering Institute where it was backcrossed onto a non-specified C57BL/6 strain.

Which mice carry the SJL-ptprcb (LY5) allele?

The Ptprcb (Ly5.2) allele is expressed by the C3H, DBA/2, and AKR strains as well as the C57BL/6. Taconic Biosciences offers a B6.SJL- Ptprca /BoAiTac congenic mouse which carries the SJL allele for Ptprca.