What are the effects of non-B DNA formation?
In the cell, non-B DNA loci can affect several DNA metabolic processes, and both replication-dependent and independent pathways contribute to elevated mutation rates at such loci. Replicative polymerases encounter many non-B DNA structures that act as natural impediments to DNA synthesis elongation.
What is conformational change in DNA?
Abstract. Both Proteins and DNA undergo conformational changes in order to form functional complexes and also to facilitate interactions with other molecules. These changes have direct implications for the stability and specificity of the complex, as well as the cooperativity of interactions between multiple entities.
Is there a link between non-B DNA formation and disease?
Current genome-wide sequence analyses suggest that the genomic instabilities induced by non-B DNA structure-forming sequences not only result in predisposition to disease, but also contribute to rapid evolutionary changes, particularly in genes associated with development and regulatory functions.
Which circumstances allow the formation of non B-DNA?
The formation of non-B-DNA within certain sequence elements of DNA can be induced by changes in environmental conditions, protein binding and superhelical tension. Several lines of evidence indicate that alternative DNA structures exist in prokaryotic and eukaryotic cells.
Which type of RNA is least stable?
mRNA
The other forms like mRNA and tRNA are synthesised whenever there is a requirement of the specific protein. mRNA is least stable and tRNA is less stable than rRNA due to its constitution and unstructured structure.
What is a conformational change mutation?
In biochemistry, a conformational change is a change in the shape of a macromolecule, often induced by environmental factors. Its shape can change in response to changes in its environment or other factors; each possible shape is called a conformation, and a transition between them is called a conformational change.
What is the difference between confirmation and conformation?
The two words do have similar religious meanings though. Conformation refers to complying with the rules of a church, while confirmation means a person’s full entry into the Christian belief.
Which of the following is not associated with the DNA?
DNA does not contain uracil. RNA contains Uracil in place of Thymine, which is the pyrimidine base of DNA.
Which conditions Favour the A and Z conformations of DNA?
A-DNA is favored by low hydration, whereas Z-DNA can be favored by high salt. The second condition is the DNA sequence: A-DNA is favored by certain stretches of purines (or pyrimidines), whereas Z-DNA can be most readily formed by alternating purine-pyrimidine steps.
What is a cruciform in biology?
Cruciform DNA is a secondary structure on a helical double-stranded DNA molecule that comprises a four-way junction and two closed hairpin-shaped points.
Is B-DNA left or right handed?
The familiar Watson-Crick double helix of DNA, called B-DNA, winds in a right-handed direction. But like a screw, the helix can wind the other way: under certain conditions, DNA adopts a left-handed form, called Z-DNA.