What is a distributive enzyme?
Distributivity means that at most one modification (addition or removal of phosphate) takes place in each encounter between substrate molecule and enzyme molecule (14). Accordingly, each phospho-form competes for both enzymes.
Which enzyme increases dystrophy of muscle?
A creatine phosphokinase (CPK) determination is the most specific test for muscular dystrophy (MD). Elevated CPK levels are indicative of muscle disease. Because the concentration of CPK is not significant in red blood cells, CPK levels are not affected by hemolysis.
Why is processivity important in DNA replication?
Processivity is defined as the ability of DNA polymerase to carry out continuous DNA synthesis on a template DNA without frequent dissociation. A common processivity factor, clamp protein or clamp, can be found in prokaryotes, eukaryotes and archaea, attesting its essential role in replication of the DNA genome.
What is distributive synthesis?
What is the difference between Distributive and Processive synthesis? Distributive–after each new base addition, the enzyme falls off the template. Processive–after each new base addition, the enzyme moves along one base on the template without falling off. Draw the reaction of DNA Pol. See slide.
What are processive enzymes?
In molecular biology and biochemistry, processivity is an enzyme’s ability to catalyze “consecutive reactions without releasing its substrate”. For example, processivity is the average number of nucleotides added by a polymerase enzyme, such as DNA polymerase, per association event with the template strand.
What do high muscle enzymes indicate?
If you have higher than normal CK-MM enzymes, it may mean you have a muscle injury or disease, such as muscular dystrophy or rhabdomyolis. If you have higher than normal CK-MB enzymes, it may mean you have an inflammation of the heart muscle or are having or recently had a heart attack.
What are the symptoms of high muscle enzymes?
Rhabdomyolysis can cause muscle pain and weakness. Blood levels of muscle enzymes, including CPK, SGOT, SGPT, and LDH, as well as blood and urine myoglobin are used to diagnose and monitor rhabdomyolysis. Hospitalization is sometimes required to treat rhabdomyolysis.
What is a processive enzyme?
Why is DNA synthesis irreversible?
Enzymatic hydrolysis of the resulting pyrophosphate into inorganic phosphate consumes a second high-energy phosphate bond and renders the reaction effectively irreversible. In general, DNA polymerases are highly accurate, with an intrinsic error rate of less than one mistake for every 107 nucleotides added.