What is a myc tag used for?
The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C-terminus of a protein.
How many kDa is myc?
The c-Myc gene encodes a polypeptide with a predicted molecular weight of 49 kDa but also demonstrates aberrant electrophoretic mobility in western blotting to manifest an apparent molecular weight of around 62 kDa (p62c-myc) [7].
How big is the myc tag?
Description
| Target: | c-Myc |
|---|---|
| Product Type: | Peptide |
| Size: | 5 x 1 mg |
| Research Area / Disease: | Epitope Tag |
| Gene ID Human: | 4609 |
What is Flag tag sequence?
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). A FLAG-tag can be used in many different assays that require recognition by an antibody.
Is c-myc the same as Myc?
The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL), and n-myc (MYCN). c-myc (also sometimes referred to as MYC) was the first gene to be discovered in this family, due to homology with the viral gene v-myc.
How do I clean myc tag protein?
Purifying Myc-tagged proteins using immunoprecipitation Immunoprecipitation (IP) is a technique for separating a protein of interest from a solution containing thousands of different proteins, such as a cell lysate, by precipitating it from the solution using an antibody that binds specifically to that protein.
Is C-MYC the same as Myc?
What is a 3X FLAG tag?
General description. The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).
How do you use a flag tag?
Additionally it can be implemented in a tandem formation, meaning multiple Flag-tags can be used right next to each other (-DYKDDDDK-DYKDDDDK-DYKDDDDK)….2. Alternatives to the Flag®-tag.
| Features of the FLAG® tag: | |
|---|---|
| Compatibility to recombinant proteins | Can be added either to the N- or C-terminus of a protein |
Is C-MYC a tumor suppressor gene?
Induced overexpression of the c-Myc gene is responsible for many of the changes that induce malignant changes (see Figure 1). These changes support the production of intermediates for cell growth and division, and are regulated by both oncogenes and tumor suppressor genes in a number of key cancer-producing pathways.
Is Myc a tumor suppressor gene?
The tumor suppressor p53 negatively regulates a number of genes, including the proto-oncogene c-Myc, in addition to activating many other genes. One mechanism of the p53-mediated c-Myc repression may involve transcriptional regulation.