What is Alexa Fluor used for?
Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules.
What does Alexa Fluor 568?
We offer Alexa Fluor 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection….Alexa Fluor 568 reactive dyes.
| Amine | Thiol | Aldehyde, ketone, carboxylate, or phosphate |
|---|---|---|
| NHS ester | Maleimide | Cadaverine Hydrazine |
Is 633 far-red?
Alexa Fluor® 633 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. The resulting Alexa Fluor® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Is Alexa 555 red?
Alexa Fluor® 555 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red-orange fluorescent Alexa Fluor® 555 dye.
What color is Alexa Fluor 555?
yellow
About Alexa Fluor 555 Alexa Fluor® 555 (AF555, Alexa 555) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter.
What Colour is Alexa Fluor 546?
orange
Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines.
What color is Alexa Fluor 680?
red
About Alexa Fluor 680 Alexa Fluor® 680 (AF680, Alexa 680) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 695/40 nm bandpass filter.
Do you wash after secondary antibody?
After incubating with the secondary antibody, the membrane is then washed with TBST or PBST buffer for 4-5 times, about 5 minutes each time with agitation, to remove residual unbound secondary antibody.
Can secondary antibodies be reused?
Finally, very few researchers will reuse secondary antibodies, as you generally burn through fewer of them per equivalent volume. Secondary antibodies are also cheaper and used at far more dilute ranges.
What is Alexa Fluor 532?
Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
What is the peak excitation of Alexa Fluor 680 dye?
With a peak excitation at 679 nm and maximum emission at 702 nm, the Alexa Fluor 680 dye is spectrally similar to the Cy5.5 dye.
How are Alexa Fluor dyes conjugated?
Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared.
What is Alexa Fluor 488 used for?
For example, Alexa Fluor 488, a sulfonated and otherwise chemically modified form of fluorescein, was designed to solve the well known issues of rapid photobleaching and pH-dependent fluorescent intensity characteristic of the dye fluorescein isothiocyanate. This section needs additional citations for verification.