What is an oligonucleotide used for?
Oligonucleotides are used as probes for detecting specific sequences that are complementary to the oligonucleotides. When a certain sequence needs to be detected, a complementary oligonucleotide is synthesized in the laboratory.
How do you purify an oligonucleotide?
High-performance liquid chromatography (HPLC) High-performance liquid chromatography (HPLC) is an efficient method for the analysis and purification of synthetic oligonucleotides. It can be used for the separation of oligomers of similar size and is amenable to automation.
What are desalted primers?
Salt-free (“desalted”) oligos are suitable for applications such as microarrays, sequencing or qPCR primers. Final QC specifications for custom-synthesized oligos are such that the observed mass must be within ±0.1% difference from the theoretical mass, as determined by electrospray mass spectrometry.
What is the difference between a polynucleotide and an oligonucleotide?
Oligonucleotides, such as RNA, are shorter molecules, containing about 20 nucleotide monomers, while polynucleotides, such as DNA, are longer and, in fact, the longest in the living world. The functions of polynucleotides are many, including coding for genes.
Are oligonucleotides hydrophobic?
Conventional lipid-conjugated oligonucleotides are amphiphilic molecules composed of two segments: negatively charged oligonucleotides as the hydrophilic moieties and lipids as the hydrophobic groups19.
How do you Desalt oligonucleotides?
7. Elute the desalted oligonucleotide by flushing the cartridge with 1 mL 50% Acetonitrile/water containing 0.5% ammonium hydroxide. The product should elute fully in 1 mL of 50% Acetonitrile with 0.5% ammonium hydroxide.
Do primers need to be purified?
Using standard primers from ABI, there is no need to purify through HPLC.
What are polynucleotides in biology?
A polynucleotide molecule is a biopolymer composed of 13 or more nucleotide monomers covalently bonded in a chain. DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are examples of polynucleotides with distinct biological function.
What is the difference between a polynucleotide and an oligonucleotide quizlet?
Oligonucleotides, such as RNA, are shorter molecules, containing about 20 nucleotide monomers, while polynucleotides, such as DNA, are longer and, in fact, the longest in the living world. Some DNA molecules in humans contain hundreds of millions of nucleotides.