What is autoMACS rinsing solution?

Rinsing Solution is used for the preparation of an optimal, preservative-free MACS Separation Buffer that can be used for cell isolation on an autoMACS Separator and all manual MACS Cell Separation Systems. The autoMACS Rinsing Solution is not suited for autoMACS Pro Separator rinsing or cleaning programs.

What is autoMACS?

Automated cell isolation with the autoMACS® Pro Separator provides fast and gentle isolation of any cell type. Proven in thousands of peer-reviewed publications, the autoMACS Pro is the most trusted automated magnetic cell separation instrument available.

How long is MACS buffer Good For?

21 days. The expiration date is indicated on the bottle label. autoMACS® Instruments are designed for automated magnetic sorting of cell samples employing MACS® Technology. Prior to usage an autoMACS Separator is primed with MACS Separation Buffer (autoMACS Running Buffer).

How do I create a Mac buffer?

Buffer: Prepare a solution containing phosphate-buffered saline (PBS) pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS BSA Stock Solution (# 130-091-376) 1:20 with autoMACS® Rinsing Solution (# 130-091-222). Keep buffer cold (4−8 °C).

What is a Mac separator?

MACS Separators are strong permanent magnet that enable the isolation of any cell type. Together with MACS MicroBeads and MACS Columns, they deliver target cells with high purities and recoveries. Thereby, even cells that are only slightly labeled with MACS MicroBeads are retained in the column.

What are LS columns?

LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells. LS Columns can be used with the following separators: MidiMACS™ Separator. QuadroMACS™ Separator.

What is MACS buffer for?

MACS Buffers and Solutions for cell isolation applications Whether for rinsing and cleaning of an instrument’s fluidic system or for buffering cell separations, the sterile solutions are made to ensure that subsequent cell separations are of the highest purity possible.

How do you split the Dock on a Mac?

Go to the Applications folder and drag the Dock Spacer app to the Dock. Drag the custom Dock spacer wherever you want to position it on the Dock. To remove the spacer, drag it off the Dock or right-click it and select Remove from Dock. Thank you for signing up!

How do I separate apps on my Mac Dock?

Add, remove, or rearrange Dock items Add an item to the Dock: Drag apps to the left side of (or above) the line that separates the recently used apps. Drag files and folders to the right side of (or below) the other line that separates recently used apps.

What is output of ls?

The output from ls –l summarizes the most important information about the file on a single line. If the specified pathname is a directory, ls displays information about every file in that directory (one file per line).

What is P in ls output?

The -p option adds a / at the end of directories, it helps you easily detect which one of the outputs is a directory and which one is a file. A similar option to -p is -F which uses these characters to indicate: / –> directories. @ –> symbolic links.

What is the purpose of the automacs rinsing solution?

How do I use the automacs® separator?

Employing the MACS® Technology, the autoMACS Separator is designed for positive selection as well as depletion of magnetically labeled cells. Using the autoMACS Separator is simple: Add the magnetic labeling reagent to a cell suspension and choose an application program from the autoMACS Touchscreen.

How many cells can the automacs separator sort?

With the autoMACS Separator, rare cells can be enriched up to 10,000- fold, or unwanted cells are depleted up to 99%. MACS magnetic cell sorting does not alter the ﬂ ow cytometric or functional properties of cells. For speciﬁ c magnetic labeling a wide range of MACS MicroBeads and MACS Antibodies is available.