What is Coomassie blue r250?
Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. …
What is the difference between Coomassie r250 and g250?
Like R-250, Coomassie G-250 (also known as colloidal Coomassie dye) also offers relatively high sensitivity and involves a simple protocol. However, G-250 offers a faster staining protocol and eliminates the need for destaining the gel (you can easily visualize the protein bands against the light amber background).
What is the difference between Coomassie Brilliant Blue G-250 and Coomassie brilliant blue r250?
Coomassie brilliant blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie brilliant blue G-250 differs from Coomassie brilliant blue R-250 by the addition of two methyl groups.
How do you cook Coomassie brilliant blue r250?
Dissolve 0.25 g of Coomassie Brilliant Blue R-250 in 90 ml of methanol:H2O (1:1, v/v) and 10 ml of glacial acetic acid. Filter the solution through a Whatman No. 1 filter to remove any particulate matter. Store at room temperature.
How does Coomassie G-250 interact with proteins?
Coomassie R-250 is the more commonly used and sensitive of the two. In the staining reaction, the Coomassie dye binds to proteins through ionic interactions between sulfonic acid groups and positive protein amine groups through Van der Waals attractions.
What does Coomassie Blue stain show?
Coomassie Blue stain is used to stain the protein bands in polyacrylamide gels. One common way to use it is to dissolve the dye in a mixture of methanol, acetic acid, and water. This stain will permeate the gel, stain the protein, and also fix the protein in place.
How does Coomassie Brilliant Blue G-250 bind to a protein?
Currently, there are two kinds of the Coomassie dyes: R-250 and G-250. In the staining reaction, the Coomassie dye binds to proteins through ionic interactions between sulfonic acid groups and positive protein amine groups through Van der Waals attractions.
Can Coomassie Brilliant Blue interfere in SDS PAGE?
Hi Aswin CBB250 is usually used to stain proteins, while bromophenol blue (BPB) is used as a tracking dye on SDS PAGE, because it does not bind proteins. Having both dyes in a gel is a bad idea.
Does Coomassie stain all proteins?
The most common used protein stain is Coomassie Blue staining, which is based on the binding of Coomassie Brilliant Blue, which binds non-specifically to virtually all proteins.
What is the purpose of the Coomassie stain?
What is the molecular weight of Coomassie Brilliant Blue G-250?
854.0
Coomassie Brilliant Blue G
| PubChem CID | 61363 |
|---|---|
| Molecular Formula | C47H48N3NaO7S2 |
| Synonyms | UNII-M1ZRX790SI 6104-58-1 Brilliant Blue G Brilliant blue G-250 M1ZRX790SI More… |
| Molecular Weight | 854.0 |
| Parent Compound | CID 61364 |
How much protein do you need for Coomassie stain?
For standard Coomassie staining, I generally load about 2ug of a singular purified protein per well. You should get a crisp dark band. In my experience, if you go much over 5ug your band will start to become more blob and less band.