What is FLP recombinase used for?
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo.
What does Cre recombinase mean?
recombination
Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites).
What is recombinase protein?
Recombinase A (RecA), a naturally aggregating protein involved in DNA repair, is an important asset to the genetic integrity of the Escherichia coli (E. coli) genome. The survival of all species rely on such DNA repair processes. Rad51, for example, is a RecA homologue found specifically in humans.
How does FRT Flippase work?
When there are two FRTs sequences in tandem, after inducing flippase the DNA sequence that is between these two sites will be deleted. If two FRT sites are facing each other (or looking away), the DNA that sits in between them can be inverted after induction of flippase.
What organism does the FRT FLP system originated from?
The FLP-FRT system. The FLP-FRT system is similar to the Cre-lox system and is becoming more frequently used in mouse-based research. It involves using flippase (FLP) recombinase, derived from the yeast Saccharomyces cerevisiae (Sadowski 1995).
What type of recombinase is Cre recombinase?
Type I topoisomerase
Cre Recombinase is a Type I topoisomerase. Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites (1).
Who discovered Cre recombinase?
Nat L. Sternberg
The origins of this tool date to more than 30 years ago when Nat L. Sternberg discovered the recombinase, Cre, and its specific locus of crossover, lox, while studying the maintenance of bacteriophage P1 as a stable plasmid.
Where is recombinase found?
Bacteria. RecA recombinase appears to be universally present in bacteria. RecA has multiple functions, all related to DNA repair. RecA has a central role in the repair of replication forks stalled by DNA damage and in the bacterial sexual process of natural genetic transformation.
Which of the following is a recombinase?
Explanation: Recombinase is a tetrameric protein that binds to the two chromosomes undergoing site-specific recombination reaction. It is a must for the reaction. Explanation: Site specific recombination in bacterial DNA and viral DNA results in a single circular chromosome.
Where is Flippase found?
the membrane
Flippases (rarely spelled flipases) are transmembrane lipid transporter proteins located in the membrane which belong to ABC transporter or P4-type ATPase families.
What does CRE mean in mice?
It has been successfully applied in yeasts, plants, mammalian cell cultures and mice (Araki et al. 1987). It is based on the ability of the P1 bacteriophage cyclization recombination (Cre) recombinase gene (cre) to effect recombination between pairs of loxP sites.
What is FLP-FRT recombination?
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo.
Which promoters are available in the FLP-in expression vectors?
Flp-In™ expression vectors are available with the CMV or EF-1α promoter. The pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO® vectors contain the human EF-1É promoter for driving expression of the gene of interest.
Can Flp recombinase be utilized in mice in a similar way?
Thus, the authors of Dymecki et al. (1998) theorized that FLP recombinase could be utilized in a similar fashion with a similar efficacy as Cre recombinase in mice. The authors created a two transgenic mouse lines: a neuronal Wnt1::Flp fusion line and a line that possessed the FRT cassette flanking the 18th exon of tm1Cwr.
What is the function of the FLP protein?
The Flp protein, much like Cre, is a tyrosine family site-specific recombinase. This family of recombinases performs its function via a type IB topoisomerase mechanism causing the recombination of two separate strands of DNA. Recombination is carried out by a repeated two-step process.