What is genetic transfection?
Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.
What does the word transfection mean?
infection
Definition of transfection : infection of a cell with isolated viral nucleic acid followed by production of the complete virus in the cell also : the incorporation of exogenous DNA into a cell.
What is the difference between transduction and transfection?
Transfection is the process of introducing nucleic acids into cells by non-viral methods. Transduction is the process whereby foreign DNA is introduced into another cell via a viral vector. A common way to validate that a genetic material was successfully introduced into cells is to measure protein expression.
What is transformation in biology?
transformation, in biology, one of several processes by which genetic material in the form of “naked” deoxyribonucleic acid (DNA) is transferred between microbial cells. Its discovery and elucidation constitutes one of the significant cornerstones of molecular genetics.
What is transfection in cell culture?
Transfection Methods for Cell Culture. Transfection is generally defined as the process of introducing DNA, RNA or proteins into cells to influence their genotype or phenotype. Besides basic research, these include gene therapy, immunotherapy and induced pluripotent stem cell generation.
Who discovered transfection?
This technique was first described in 1984 by Tsukakoshi et al., who used a frequency tripled Nd:YAG to generate stable and transient transfection of normal rat kidney cells. In this technique, one cell at a time is treated, making it particularly useful for single cell analysis.
How does transfection work?
Transfection. Transfection can be carried out using calcium phosphate (i.e. tricalcium phosphate ), by electroporation, by cell squeezing or by mixing a cationic lipid with the material to produce liposomes that fuse with the cell membrane and deposit their cargo inside.
What is transfection of cells?
In animal cells, transfection is the preferred term as transformation is also used to refer to progression to a cancerous state (carcinogenesis) in these cells. Transduction is often used to describe virus-mediated gene transfer into eukaryotic cells.
What is RNA transfection?
RNA transfection is the process of deliberately introducing RNA into a living cell. RNA can be purified from cells after lysis or synthesized from free nucleotides either chemically, or enzymatically using an RNA polymerase to transcribe a DNA template.
What is bacterial transfection?
Transduction, a process of genetic recombination in bacteria in which genes from a host cell (a bacterium) are incorporated into the genome of a bacterial virus (bacteriophage) and then carried to another host cell when the bacteriophage initiates another cycle of infection.