What is goat anti mouse IgG?
Goat anti Mouse IgG (H/L) polyclonal antibody reacts with the heavy chains of mouse IgG, and the light chains common to the majority of mouse immunoglobulins. No antibody reactivity was detected against non-immunoglobulin mouse serum proteins. This antibody may cross-react with immunoglobulins from other species.
What is goat anti mouse antibody?
Anti-mouse secondary antibodies are generated by immunizing a host animal (e.g., a goat) with a pooled population of immunoglobulins (Ig) from the target species (i.e., mice). These antibodies are designated as cross-adsorbed or highly cross-adsorbed, based on the amount of processing which has occurred.
What is mouse IgG?
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG is the main antibody isotype found in blood and extracellular fluid allowing it to control infection of body tissues.
Is Alexa Fluor an antibody?
Alexa Fluor® 488 secondary antibody is ideal immunofluorescence antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy.
Can mouse antibodies be used in humans?
Human anti-mouse antibodies (HAMA) are human immunoglobulins with specificity for mouse immunoglobulins. This topic currently is of interest because of the increased use of monoclonal mouse antibodies as diagnostic reagents both for in vitro laboratory measurements and for in vivo imaging studies.
Can I use a mouse antibody on mouse tissue?
Much of the background is caused by secondary antibody binding to endogenous mouse IgG in the tissue being stained, and to Fc receptors on B cells, plasma cells and macrophages. Abcam offers a robust kit to allow use of mouse antibody on mouse tissue.
What is the difference between a primary and a secondary antibody?
The primary antibody has the ability to bind directly to the antigen whilst a secondary antibody doesn’t bind to the antigen directly but interacts through the binding to a primary antibody. This is the key difference between primary and secondary antibody.
What is irrelevant IgG?
The term “irrelevant” refers to a primary antibody of the same isotype as the specific primary antibody (i.e. mouse IgG) and applied at the same concentration, that is known not to bind to a target in the tissue specimen.
How are IgG antibodies produced?
IgG antibodies are generated following class switching and maturation of the antibody response, thus they participate predominantly in the secondary immune response. IgG is secreted as a monomer that is small in size allowing it to easily perfuse tissues.