What is MSTFA used for?
N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) is a very versatile reagent because it can be used to derivatize many of the functional groups commonly found in organic compounds. It operates by attachment of a nonpolar trimethylsilyl (TMS) group (Figure 1).
What is silylation reaction?
Silylation is the introduction of a (usually) substituted silyl group (R3Si) to a molecule. The process is the basis of organosilicon chemistry.
What is Bstfa?
BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) is the pre- ferred reagent for trimethylsilylation of alcohols, alkaloids, amines and biogenic amines, carboxylic acids, phenols, and steroids.
What is Bstfa derivatization?
BSTFA + TMCS as a Derivatization Reagent for GC Derivatization is a technique that usually involves a reaction of the analyte(s) of interest with a particular derivatization reagent. The reagent N,Obis( trimethylsilyl)trifluoroacetamide (also called BSTFA) is regularly used for this reaction.
What is silylation reagent?
In silylation, an active hydrogen is replaced by an alkylsilyl group, such as trimethylsilyl (TMS), or t-butyldimethylsilyl (t-BDMS). Compared to their parent compounds, silyl derivatives are more volatile, less polar, and more thermally stable.
Why is GC derivatized?
The derivatization is typically done to change the analyte properties for a better separation and also for enhancing the method sensitivity. In GC/MS, derivatization may improve the capability of compound identification. Examples illustrating such improvements are included.
How does derivatization work?
Derivatization is a technique used in chemistry which converts a chemical compound into a product (the reaction’s derivate) of similar chemical structure, called a derivative. Resulting new chemical properties can be used for quantification or separation of the educt.
What is derivatization reagent?
Derivatization reagent is the substance that is used to chemically modify a compound to produce a new compound which has properties that are suitable for analysis in GC or LC. The reagent should produce more than 95 % complete derivatives.
What is derivatization in GC analysis?
Derivatization is the process by which a compound is chemically changed, producing a new compound that has properties more. amenable to a particular analytical method. Some samples analyzed by GC require derivatization in order to make them suitable for. analysis.
How do you Deprotect silyl ether?
Typical deprotection protocols for the removal of silyl ethers are acidic aqueous THF, or acidic methanol, alkaline aqueous solutions and sources of fluoride ion, most commonly tetra-n-butylammonium fluoride, TBAF, in various solvents.
Which base is used for preparation of silyl ether?
The most common method for attaching this group reacts the alcohol with tert-butyldimethylsilyl chloride using imidazole65a or dimethylaminopyridine65b as the base. The analogous silyl triflate (t-BuMe2SiOSO2CF3) is also useful for generating this ether from alcohols, with an amine base.
How do you increase the silylation power of MSTFA?
The silylation power of MSTFA can be enhanced by the use of catalysts or additives that scavenge reaction by-products. MSTFA reacts in situ with ammonium iodide (NH 4 I) to produce trimethyliodosilane (TMSI), a powerful trimethylsilyl donor. 5
MSTFA (see Figure 1 for the molecular structure) is an effective trimethylsilyl donor similar to BSA and BSTFA.1 MSTFA reacts to replace labile hydrogens on a wide range of polar compounds with a -Si(CH3) 3 group (Figure 2)and is used to prepare volatile and thermally stable derivatives for gas chromatography and mass spectrometry.
What is the reaction between MSTFA and ethanethiol?
MSTFA reacts in situ with ammonium iodide (NH 4 I) to produce trimethyliodosilane (TMSI), a powerful trimethylsilyl donor. 5 In order to prevent iodine incorporation into the product, ethanethiol is added to reduce the formed iodine to hydrogen iodide. As a result, diethyl disulphide is produced during the derivatization reaction.
How does MSTFA convert ezetimibe to TMS?
In this derivatization reaction, MSTFA reacts with labile hydroxyl group of ezetimibe, and the hydroxyl groups are then converted into TMS–ether derivatives under trimethylsilylation derivatization conditions.