What is myc antibody?
The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C-terminus of a protein.
What is the size of MYC?
Description
| Target: | c-Myc |
|---|---|
| Size: | 5 x 1 mg |
| Research Area / Disease: | Epitope Tag |
| Gene ID Human: | 4609 |
| Gene ID Mouse: | 17869 |
What result does C-MYC have on transcription?
In cancer, c-myc is often constitutively (persistently) expressed. This leads to the increased expression of many genes, some of which are involved in cell proliferation, contributing to the formation of cancer….Myc.
| MYC proto-oncogene, bHLH transcription factor | |
|---|---|
| Identifiers | |
| NCBI gene | 4609 |
| HGNC | 7553 |
| OMIM | 190080 |
What is the purpose of a myc tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
What is a V5 tag?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What are Myc tagged proteins?
What is the difference between C-Myc and N MYC?
c-Myc is broadly overexpressed in both blood-borne and solid tumors. N-myc is most frequently overexpressed in solid cancers of neural origin, such as neuroblastoma and glioma.
Is MYC a tumor suppressor?
More importantly, through the induction of miR-145, p53 is able to repress c-Myc expression at the posttranscriptional level. Therefore, our finding identifies a critical role of miR-145 as a tumor suppressor in the p53 regulatory network and thus provides new insight into the p53-mediated regulation of c-Myc.
How many kDa is Myc?
The c-Myc gene encodes a polypeptide with a predicted molecular weight of 49 kDa but also demonstrates aberrant electrophoretic mobility in western blotting to manifest an apparent molecular weight of around 62 kDa (p62c-myc) [7].
What is a Myc DDK tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
Where is the V5 antigen located?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. The V5 tag is generally used with all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence.
What is antiantimyc/c-Myc antibody (9e10)?
Anti-Myc/c-Myc Antibody (9E10) is rated 4.7 out of 5 by 243 . raised against an epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin
Are monoclonal antibodies specific for MYC more context sensitive?
We conclude that recently developed, purpose-made monoclonal antibodies specific for Myc have much more uniform reactivity in diverse assays and are much less context sensitive than is the legacy antibody 9E10. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
Which myc-tag antibody has the highest context-dependent detection variability?
In particular, clone 9E10, the most cited Myc-tag antibody, displayed high context-dependent detection variability, whereas others, notably 4A6 and 9B11, showed much less context sensitivity in their detection of Myc-tagged proteins. The very high context sensitivity of 9E10 was further substantiated by peptide microarray analyses.
What does 9e10 stand for?
Anti-Myc/c-Myc Antibody (9E10) is a mouse monoclonal IgG 1 κ Myc/c-Myc antibody, cited in 8,987 publications, provided at 200 µg/ml. raised against an epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin. This Myc antibody is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of