What is NGAL test?
The NGAL TestTM is a particle-enhanced turbidimetric immunoassay for the quantitative determination of neutrophil gelatinase-associated lipocalin (NGAL) in human EDTA plasma for testing on automated clinical chemistry analyzer.
What is neutrophil gelatinase-associated lipocalin?
Neutrophil gelatinase-associated lipocalin (NGAL) is a polypeptide released by damaged nephron tubular cells in the setting of local inflammation. 64. In multiple settings, NGAL has been shown to be identifiable in the serum and urine within 1–4 hours of ischemic renal insult and highly predictive of AKI.
Where is NGAL produced?
Neutrophil gelatinase-associated lipocalin (NGAL) is produced in injured kidney. NGAL has been used as an early plasma biomarker for AKI in patients undergoing heart surgery. Neutrophils contain all isoforms (25-kDa, 45-kDa and 145-kDa) but the kidney produces almost exclusively the 25-kDa isoform of NGAL.
What is NGAL biomarker?
Neutrophil gelatinase-associated lipocalin (NGAL) is released from kidney tubular cells under stress as well as from neutrophils during inflammation. It has been suggested as a biomarker for acute kidney injury (AKI) in critically ill patients with sepsis.
How early can urine NGAL be detected in both urine and blood after a tubular injury?
Conclusion. NGAL as diagnostic marker: Elevated NGAL level as an early blood-based marker of AKI is detectable within 2-12 hours after an ischemic/toxic insult in patients with cardiovascular diseases. Although NGAL in urine and in serum have a somewhat different origin, both are useful for early diagnosis of AKI.
How do you interpret Cystatin C?
Cystatin C is a protein that is produced by the cells in your body. When kidneys are working well, they keep the level of cystatin C in your blood just right. If the level of cystatin C in your blood is too high, it may mean that your kidneys are not working well.
What is kim1?
Kidney Injury Molecule-1 (KIM-1) is a type 1 transmembrane protein, with an immunoglobulin and mucin domain, whose expression is markedly up-regulated in the proximal tubule in the post-ischemic rat kidney. The ectodomain of KIM-1 is shed from cells.
What is the role of NGAL in the diagnosis of Aki?
Neutrophil gelatinase-associated lipocalin (NGAL) is an emerging biomarker for the early detection of AKI. Studies have shown NGAL to be a highly induced protein in the kidney after ischemic or nephrotoxic injury.
How much does the NGAL test cost?
The average cost per test of The NGAL Test is estimated to be £24, assuming the test reagent kit, controls and standards can be used for about 100patients. The individual components needed for. the test are The NGAL Test Reagent kit (£1770), the calibrator kit (£213) and the control kit (£417).
What is the normal range of NGAL in urine?
Using similar methodology, a reference interval for urine NGAL in adults was determined to be < 113 ng/mL. Age- and sex-specific ranges have not been established, but there is evidence that urine NGAL concentrations increase with age and are higher in females than in males.
What does it mean if your NGAL is over 300?
Elevations of urine NGAL are not entirely kidney specific, and can also be observed with infections, inflammation, and in some cancer patients. Urine levels greater than 300 ng/ml yield a higher specificity Collect a random urine specimen. Refrigerate for up to 3 days. Any additive.