What is Ollas tag?
The OLLAS (E.coli OmpF Linker and mouse Langerin fusion Sequence) tag is a new, highly sensitive epitope tag. OLLAS tags are detectable using the OLLAS monoclonal antibody at approximately 100-fold lower levels than similar commercially available tags, including Sigma’s FLAG™ epitope tag system.
How do epitope tags work?
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The fusion protein can then be detected and/or purified using a monoclonal antibody specific for the epitope tag.
What is V5 tagging?
V5 tag is a short peptide tag for detection and purification of proteins. The V5 tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.
How do I choose an epitope tag?
Choice of an epitope tag depends on many factors, but downstream application is the most important one. If the desired downstream application is recombinant protein purification, epitope tags like 6X-His, GST (Glutathione-S-transferase), and MBP (Maltose Binding Protein) are widely used in affinity purification.
What is the V5 epitope?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is myc DDK tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
What is HA epitope tag?
The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is Ty epitope tag?
The TY1 tag is a 10-residue peptide epitope with the amino acid sequence, EVHTNQDPLD. The epitope may also be used in repeat sequences, such as with 2x-TY1 or 3x-TY1. TY1 antibodies may be useful tools in the immunological detection or purification of TY1-tagged fusion proteins.
Where does the protein tag go?
If protein is multimeric, undergoes post translational modification with cleavage at N, C terminals. Then do tag at either N terminal or C terminal purify protein.