What is the function of pectinase?
Pectinase is used in the extraction, clarification, filtration, and depectinization of fruit juices and wines by enzymatically breaking down the cell wall, and for the maceration of fruits and vegetables and removal of the inner wall of lotus seed, garlic, almond, and peanut.
What is pectinase and why is it used in the food industry?
Pectinases contains groups of enzymes that degrade the pectin substances. In food sector, these enzymes are used for extraction, clarification, and stabilization of fruit juices. They also increase juice yield and involved in coffee, cocoa and tea fermentation and in preparation of jams and jellies.
What is the microbial source of pectinase?
Pectinase is obtained from the microbial soruce Sclerotina libertine.
Which of the fungi are used for pectinase production?
Aspergillus niger
Aspergillus niger is the most commonly used fungal species for industrial production of pectinolytic enzymes.
What is the structure of pectinase?
Pectinase possesses a complicated enzymatic system responsible for the degradation of pectic substances like pectinic acids, propectins, pectins, and pectic acids (7). According to the enzymatic nomenclature system, pectinase hydrolyses the pectin polymer more efficiently than other pectinase groups of enzymes.
How do you extract pectinase?
After production of enzyme, it is easily extracted by using Whatman filter-paper 4. Biomass stays on the filter paper and liquid as supernatant passes through it [16]. The broth is centrifuged for measuring activity of pectinase [17].
How do you make pectinase enzyme?
The bananas were washed under tab water and peeled with hand. The peels were then dried at 45 °C for 24 h and then grinded using commercial grinder and passed through 200 mesh sieve to obtain banana peel powder. The powder thus produced was then used as substrate for the production of Pectinase enzyme.
What is the substrate of pectinase?
Pectinase is a general term for enzymes, such as pectin lyase, pectin methylesterase and polygalacturonase, commonly referred to as pectic enzymes. These break down pectin, a polysaccharide substrate that is found in the cell walls of plants.
How do you make pectinase?
Make a 10% solution of pectinase enzyme by adding 1 g of enzyme to 9 mL of distilled water. Solutions should be made fresh throughout the day. 1. Students should mass two 50 g quantities of apple chunks and place into two 200-mL beakers.
What is the product that you will measure in the pectinase experiment?
The goal of this project is to monitor enzyme activity by measuring the amount of apple juice released by pectinase.
How do you test for pectinase activity?
The most frequently used method for doing this involves a “single-time-point” assay that requires two steps. The first step is a hydrolysis step, in which the pectinase-containing extract is incubated for a fixed time under selected conditions of pH, temperature and initial pectin concentration.
What is the composition of pectinase screening agar?
Pectinase Screening Agar Medium (PSAM) is used for the selective growth of those microbes which release pectin. It was prepared as per the following composition0.3gms/100ml, (NH4)2HPO4,0.2gms/100ml-KH2PO4,0.3gms/100ml,K2HPO4,0.01gms/100ml-MgSO4, 2.5gms/100ml–Agar,1gm/100ml –Pectin and PH-4.5
What is the first step of pectinase extraction?
The first step is a hydrolysis step, in which the pectinase-containing extract is incubated for a fixed time under selected conditions of pH, temperature and initial pectin concentration. In the second step, the reducing sugars liberated in the first step are quantified, either by the DNS method [16] or by the Somogyi-Nelson method [17], [18].
What can pectinases be used for?
Pectinases could also be used for the saccharification of pectin-rich agricultural residues, with the resulting sugars being used for the production of ethanol or platform chemicals [5] – [7]. In this manner, they would play an important role in the recently proposed citrus waste biorefineries [8], [9].