What is the hypothesis in the photosynthesis lab?
It was hypothesized that if the concentration of carbon dioxide was increased, then the rate at which photosynthesis occurs will also increase. The null hypothesis was that the concentration of carbon dioxide will have no effect on the rate of photosynthesis. The results of the experiment supported the hypothesis.
What does chromatography tell us about plant pigments?
What is the Science? Leaf chromatography is an experiment that allows us to see the colorful pigments that leaves have hidden inside them. The green leaf color is the chlorophyll, which helps plants absorb the yellow and blue wavelengths of light.
What did chromatography reveal in the photosynthesis investigation?
Chromatography and Photosynthesis Chromatography has revealed that many different pigments, not only green ones, are simultaneously present in leaves. Each pigment absorbs only certain colors of light from sunlight, rather than absorbing all the incident light energy that falls upon it.
What is the theory behind paper chromatography?
The principle behind the paper chromatography is that the most soluble substances move further on the filter paper than the least soluble substances. Different plant pigments can be separated by using the technique of paper chromatography.
Which best explains the role of plant pigments in photosynthesis?
What is the main role of the pigment molecules? Absorb photons and transfer light energy to the reaction center chlorophyll. The rate of photosynthesis is much greater in the green than red light spectrum. Green and yellow wavelengths of light are useless to most plants.
Why do pigments separate in chromatography?
Pigments are separated according to differences in their relative solubilities. The V-shaped tip of the paper is placed in the chromatography solvent and acts as a wick to draw the solvent up the paper, separating pigments according to their relative solubility and molecular weights.
Why do components separate in chromatography?
The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another. The nature of the specific mobile and stationary phases determines which substances travel more quickly or slowly, and is how they are separated.
What does paper chromatography separate?
Chromatography can be used to separate mixtures of coloured compounds . Mixtures that are suitable for separation by chromatography include inks, dyes and colouring agents in food.
What properties of the photosynthetic pigments causes them to separate on the chromatography paper?
Four primary pigments of green plants can easily be separated and identified using a technique called paper chromatography. These pigments include two greenish pigments called chlorophylls and two yellowish pigments called carotenoids. Pigments are separated according to differences in their relative solubilities.
How is light and pigments important to photosynthesis?
The importance of pigment in photosynthesis is that it helps absorb the energy from light. When light energy (photons of light) falls on these pigments, the electrons absorb this energy and jump to the next energy level.