What is the order of RNA processing?
These are: Addition of a 5′ cap to the beginning of the RNA. Addition of a poly-A tail (tail of A nucleotides) to the end of the RNA. Chopping out of introns, or “junk” sequences, and pasting together of the remaining, good sequences (exons)
What are the 3 steps of RNA processing?
RNA processing in chloroplasts includes mRNA 5′- and 3′-end processing, intron splicing, and intercistronic cleavages of polycistronic messages, as well as typical tRNA and rRNA processing. These posttranscriptional steps, along with changes in RNA stability, have received considerable attention for two reasons.
How is mRNA processed in eukaryotes?
In eukaryotes, transcription produces a pre-mRNA molecule that be processed into mature mRNA by the addition of a 5′ cap, a 3′ poly-A tail, and through RNA splicing. At the end of eukaryotic transcription, a pre-mRNA molecule is formed.
What are the steps of post transcriptional processing in eukaryotes?
These modifications are 5′ capping, 3′ polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated.
Where does splicing occur in eukaryotes?
the nucleus
Splicing occurs in the nucleus before the RNA migrates to the cytoplasm. Once splicing is complete, the mature mRNA (containing uninterrupted coding information), is transported to the cytoplasm where ribosomes translate the mRNA into protein. The pre-mRNA transcript contains both introns and exons.
Which of the following is not part of RNA processing in eukaryotes?
Which of the following is not part of RNA processing in eukaryotes? Reverse transcription is the enzymatic synthesis of DNA from an RNA template by the enzyme reverse transcriptase. This enzyme is not involved in the RNA processing reactions.
Which step in mRNA processing occurs first?
In the first step, the pre-mRNA is cut at the 5′ splice site (the junction of the 5′ exon and the intron). The 5′ end of the intron then is joined to the branch point within the intron. This generates the lariat-shaped molecule characteristic of the splicing process.
Which of the following RNA is transcribed by RNA polymerase II in eukaryotes?
Eukaryotic cells contain three distinct nuclear RNA polymerases that transcribe different classes of genes (Table 6.1). Protein-coding genes are transcribed by RNA polymerase II to yield mRNAs; ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs) are transcribed by RNA polymerases I and III.
What is the sequence of a polypeptide based on the mRNA sequence 5 Uuuucuuauugucuu 3?
The sequence of the peptide, based on the 5’… UUUUCUUAUUGUCUU 3′ mRNA sequence will be Phe-Ser-Tyr-Cys-leu.
Which of the following is not a step of RNA processing?
3. Which of the following is NOT the step of mRNA processing? Explanation: mRNA processing occurs only in eukaryotes, it involves 5’capping, slicing of introns, polyadenylation, and RNA editing before transported to the cytoplasm, where they are translated to the ribosome. 4.