What is the role of the C-terminal domain of RNA polymerase II?
RNA polymerase II, and specifically the C-terminal domain (CTD) of its largest subunit, has been demonstrated to play important roles in capping, splicing, and 3′ processing of mRNA precursors. Our results provide new insights into the mechanism by which the CTD functions in splicing.
Which subunit of RNA polymerase is responsible for phosphorylation in C-terminal domain?
RNA polymerase II
Ser-5 phosphorylation of the RNA polymerase II (Pol II) C-terminal domain by TFIIH kinase has been implicated in critical steps in mRNA synthesis, such as Pol II promoter escape and mRNA 5′-capping. However, the general requirement and precise role of TFIIH kinase in Pol II transcription still remain elusive.
How many subunits are in RNA polymerase II?
12-subunit
RNA polymerase II (pol II), the enzyme responsible for all mRNA synthesis in eukaryotes, has been isolated in two forms, a 12-subunit “complete” enzyme and a 10-subunit “core.” The two additional subunits of the complete enzyme, Rpb4 and Rpb7, form a heterodimer and associate reversibly with core.
What is the function of RNA polymerase II?
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of eukaryotic cells.
What is the function of the C-terminal domain?
The C-terminal domain of RNA polymerase II functions as a phosphorylation-dependent splicing activator in a heterologous protein. Mol Cell Biol.
What is the role of the C-terminal domain of RNA polymerase II quizlet?
The function of the CTD of RNA polymerase II is to couple transcription with RNA processing, which leads to a complete mRNA message.
What does C-terminal domain do?
RNA polymerase II, and specifically the C-terminal domain (CTD) of its largest subunit, has been demonstrated to play important roles in capping, splicing, and 3′ processing of mRNA precursors.
What does RPB1 stand for?
RPB1
| Acronym | Definition |
|---|---|
| RPB1 | RNA (Ribonucleic Acid) Polymerase II |
Which is not dependent on the C-terminal domain of RNA polymerase 2?
Which of the following is not dependent on the C-terminal domain (CTD) of RNA polymerase II? Explanation: The correct answer is none of the other answers. Only mRNA transcribed by polymerase II undergo 5′ capping, polyadenylation, and splicing.
What role does the C-terminal tail play for RNA polymerase?
The C-terminal repeat domain (CTD), an unusual extension appended to the C terminus of the largest subunit of RNA polymerase II, serves as a flexible binding scaffold for numerous nuclear factors; which factors bind is determined by the phosphorylation patterns on the CTD repeats.
What aspect of the C-terminal tail is responsible for determining the different functions?
What aspect of the C-terminal tail is responsible for determining the different functions? the 3ʹ poly(A) tail and PABP bind initiation factor eIF4G, stabilizing the 5ʹ end of the mRNA.
What is the carboxy-terminal domain of RNA polymerase II?
The carboxy-terminal domain of RNA polymerase II typically consists of up to 52 repeats of the sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser. The domain stretches from the core of the RNAPII enzyme to the exit channel, this placement is effective due to its inductions of “RNA processing reactions]
What is the fourth largest subunit of RNA polymerase?
RNA polymerase II subunit B4 (RPB4) – encoded by the POLR2D gene is the fourth-largest subunit and may have a stress protective role. RPB5 – In humans is encoded by the POLR2E gene. Two molecules of this subunit are present in each RNA polymerase II. RPB5 strongly interacts with RPB1, RPB3, and RPB6.
What is required for RNA polymerase II to bind to promoters?
A wide range of transcription factors are required for it to bind to upstream gene promoters and begin transcription. RNA polymerase II of Saccharomyces cerevisiae consisting of all 12 subunits.
What is the function of RNA polymerase II holoenzyme?
RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.