What is UV Transilluminator?
An ultra-violet (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The key application for a UV transilluminator is for visualization of DNA and protein agarose and polyacrylamide gels after electrophoresis.
What happens when E coli is exposed to UV light?
Ultraviolet (UV) radiation is known to inhibit cell growth and induce gene damage (1). For these reasons, UV radiation is used as a method to sterilize surgical instruments because it kills the bacteria present and disrupts bacterial reproduction (2).
What voltage should I run my agarose gel?
4–10 volts/cm
We recommend running agarose gels at 4–10 volts/cm under horizontal electrophoretic conditions. Higher voltage may result in band streaking while lower voltage may result in reduced mobility of small (<1000 bp) DNA and diffusion.
What is the purpose of the gel in electrophoresis?
Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.
Why does EtBr light up under UV exposure?
The reason for Ethidium Bromide’s intense fluorescence after binding with DNA is the hydrophobic environment found between the base pairs. By moving into this environment and away from the solvent, the EtBr cation is forced to shed any water associated molecules.
Can E. coli survive UV light?
Radiation at 265 nm in the UV region was most efficient in killing the E. coli cells and 100% mortality was achieved at a dose of 1.17 log mJ/cm(2). In the visible spectrum, the radiation dosages required for a one-log reduction of the E. coli cell density at 458 and 488 nm were 5.5 and 6.9 log mJ/cm(2), respectively.
Why was there still E. coli growth after UV exposure How can you explain this result?
The control was the side of plate that was NOT exposed to UV light. Why was there still E. Coli still has repair mechanisms for UV radiation, which is why we saw it have growth will shorter exposure.
Is UV radiation from transilluminator dangerous?
Hazard and Risks from Transilluminator UV Radiation. It is important to note that the UV radiation used in transilluminators is harmful to both skin and eyes. UV radiation cannot be seen and. is not felt immediately; the user may not realize the danger until after the exposure has caused damage.
What wavelength bands do uvuv transilluminators operate at?
UV transilluminators operate at one of several wavelength bands, depending on the type of sample. Standard wavelength bands are 254, 312, and 365 nanometers (nm). Most of these instruments are stationary, but a few hand-held types carry the same hazards as those of the stationary models.
What is uvuv radiation and why is it dangerous?
UV radiation is often a secondary hazard from plasma. UV light can also escape from other parts of the system (e.g., inductively coupled plasma sources and downstream plasma discharge tubes). Careful filtering and shielding is required to avoid UV exposure.
What is the use of UV light in biotechnology?
UV transilluminators or UV light boxes are used in biotechnology for visualization of nucleic acids (DNA or RNA) after gel electrophoresis and ethidium bromide staining. Samples are placed on the illumination window and illuminated by UV light.