What is ZIC Hilic?
The ZIC®-HILIC Column is designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The robust bonded hydrophilic phase on ZIC®-HILIC ensures a stable environment for the HILIC partitioning process during analyte retention.
What is Hilic column?
Our broad variety of hydrophilic interaction liquid chromatography (HILIC) columns provides strong retention of compounds that are unretained under conventional reversed phase conditions, without the requirement for ion-pair additives in the mobile phase. These columns are ideal for high sensitivity LC-MS applications.
How to improve peak shape in HILIC?
By matching the injection solvent to the initial mobile phase conditions, you get better peak shape, increased retention, and higher sensitivity. Figure 2: Matching the injection solvent to the mobile phase results in better peak shape, retention, and sensitivity.
Is HILIC normal phase?
HILIC is a variation of normal phase chromatography. The major differences between HILIC and NP are the composite of the mobile phase and the mechanism of separation. Common NP chromatography uses 100 % organic mobile phases while HILIC uses organic mobile phases that are water miscible.
What is HILIC used for?
Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds.
How do you clean Hilic columns?
A suitable wash solution is 60% organic solvent in water (unless otherwise specificed in product manuals). HILIC columns should be stored under HILIC conditions. A mixture of organic solvent and water in a 90:10 ratio is recommended for storage.
What does Hilic stand for?
What is the difference between Hilic and normal phase?
What is HILIC mode?
HILIC is a high pressure liquid chromatography (HPLC) mode that combines the use of a polar column with a mobile phase composed of a high percentage of organic solvent and a low percentage of aqueous buffer, where the water phase acts as the strong solvent.
How do you clean a ZIC HILIC column?
The ZIC®-HILIC column can be heated and operated up to 70 °C. An initial washing with deionised water is used to remove organic solvent and polar impurities, followed by a flush with a 0.5 M sodium chloride solution.
How do you store a HILIC column?
HILIC columns should be stored under HILIC conditions. A mixture of organic solvent and water in a 90:10 ratio is recommended for storage. Ensure the column end-fittings are sealed with end-plugs to prevent the stationary phase from drying.
Is Hilic normal phase?
What is ZIC ® -HILIC?
The silica-based ZIC ® -HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC ® -HILIC Column overall neutral, with weak, but important, ionic interactions.
Why sequant® ZIC®-HILIC HPLC columns?
Merck’s SeQuant® ZIC®-HILIC HPLC columns offer excellent selectivity, high loadability and easy optimization for the separation of polar hydrophilic compounds. SeQuant® ZIC®-HILIC HPLC columns have documented excellent selectivity for separations of polar hydrophilic compounds in HPLC and LC-MS methods.
What is hydrophilic interaction liquid chromatography (HILIC)?
Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC.
What is HILIC separation?
Simply put, HILIC is a normal-phase type of separation but uses reversed-phase type eluents. Thus, it unites the benefits of aqueous mobile phases with separation based on normal phase principles in which more polar compounds respond with higher retention.