Which of following is site-specific recombination?
Which of the following is a must for site-specific recombination reaction? Explanation: Recombinase is a tetrameric protein that binds to the two chromosomes undergoing site-specific recombination reaction. It is a must for the reaction.
What are two types of recombinase proteins which involved in site-specific recombination SSR )?
Site-specific recombination systems are also used to control gene expression. The recombinases fall into one of two protein families, the serine and the tyrosine recombinases, named after the amino acid residues that transiently become covalently linked to the DNA substrates during recombination.
Is transposition a site-specific recombination?
Transposition is the process by which genetic elements move between different locations of the genome, whereas site-specific recombination is a reaction in which DNA strands are broken and exchanged at precise positions of two target DNA loci to achieve determined biological function.
What is the specific site for recombination by Cre enzyme?
The Cre protein encoded by the coliphage P1 is a 38-kDa protein that efficiently promotes both intra- and intermolecular synapsis and recombination of DNA both in Escherichia coli and in vitro. Recombination occurs at a specific site, called lox, and does not require any other protein factors.
Where does genetic recombination occur?
meiosis
Recombination occurs when two molecules of DNA exchange pieces of their genetic material with each other. One of the most notable examples of recombination takes place during meiosis (specifically, during prophase I), when homologous chromosomes line up in pairs and swap segments of DNA.
How does homologous recombination differ from site-specific recombination?
The key difference between homologous recombination and site-specific recombination is that in homologous recombination, genetic material is exchanged between two identical molecules of double-stranded or single-stranded nucleic acids such as DNA or RNA, whereas in site-specific recombination, DNA strand exchange takes …
What is transposition recombination?
Transpositional recombination is a process in which a mobile element is inserted into a target DNA. It may occur by one of two mechanisms: (1) directly as DNA, (2) through RNA. The mobile elements that transpose through DNA are called transposons and those via RNA are referred to as retrotransposons.
What is conservative site-specific recombination?
Conservative site-specific recombination is a process that enables genetic recombination between DNA molecules that contain short DNA sequences, which are bound by specific recombinase proteins. The outcomes of site-specific recombination can include DNA integration, deletion, or inversion.
What are the three types of recombination?
There are three types of recombination; homologous recombination, specific recombination and transposition . Each type occurs under different circumstances. Homologous recombination occurs in eukaryotes , typically during the first phase of the meiotic cell division cycle.
What is an example of recombination?
According to the this definition every DNA molecule is a recombinant DNA molecule as during meiosis crossing over with homologous recombination occurs and new recombinant DNA molecules are generated. Even bacterial cells have the examples of homologous recombination.
What is recombination answers?
Recombination is a process by which pieces of DNA are broken and recombined to produce new combinations of alleles. This recombination process creates genetic diversity at the level of genes that reflects differences in the DNA sequences of different organisms.
What is the definition of recombination?
Definition of recombination. : the formation by the processes of crossing-over and independent assortment of new combinations of genes in progeny that did not occur in the parents.