Which of the following reactions is required for proofreading during DNA replication?
Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III? Explanation: The 3′ 5′ exonuclease activity removes the mispaired nucleotide and the polymerase begins again. This activity is known as proofreading. 7.
Which of the following is involved in DNA replication?
During DNA replication, one new strand (the leading strand) is made as a continuous piece. The other (the lagging strand) is made in small pieces. DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.
Why is a primer not required for transcription?
RNA primers are needed to begin replication because DNA polymerase is unable to do it alone. DNA transcription does not have the same problem because RNA polymerase is capable of initiating RNA synthesis.
Which enzyme is not required for DNA replication?
DNA polymerase I is the enzyme that replaces the RNA nucleotides in a primer with DNA nucleotides. Which of the following is not involved in the initiation of replication? Answer a. Ligase is not involved in the initiation of replication.
What three main enzymes are needed for DNA replication?
Enzymes involved in DNA replication are:Helicase (unwinds the DNA double helix)Gyrase (relieves the buildup of torque during unwinding)Primase (lays down RNA primers)DNA polymerase III (main DNA synthesis enzyme)DNA polymerase I (replaces RNA primers with DNA)Ligase (fills in the gaps)
What is required for DNA replication?
Most organisms, including mammals, use bi-directional replication. There are four basic components required to initiate and propagate DNA synthesis. They are: substrates, template, primer and enzymes.