Why is BG 11 medium used for cyanobacteria?
This medium supports growth of photoautotrophic blue green algae (1,2). They require light as source of energy. Synthetic nitrogen and carbon sources and other inorganic salts comprise this medium. Exposure to light intensity of 2,000 to 3,000 lux is optimal for cultivation of blue green algae.
What is BG 11 medium used for?
BG11 Broth is a universal medium for the cultivation and maintenance of blue green algae (cyanobacteria).
Which media used for grow of blue green algae?
Cyanophycean Agar is used for the isolation and cultivation of blue green algae. Potassium is required for maintenance of maximum growth rate of blue green algae (3). Nitrate serves as nitrogen source. Dipotassium phosphate buffers the media.
How to prepare BG 11 medium?
Combine the following solutions and adjust pH to 7,5 (Use 1.0M HCl or NaOH). Stock Per liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0,02 g Stock 4 1,0 ml Page 2 NaNO3 1.5 g (For 100x BG-11 solution don’t add NaNO3) For solid media: add 1.5% agar to the liquid BG11 media.
How do you make bold basal medium?
The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).
Can you culture cyanobacteria?
Alternation of the agar plating and liquid culture is continued until axenic cultures are obtained. Culture collections can furnish important organisms for study. Large-scale culture of Cyanobacteria is conveniently achieved by simple continuous culture techniques in 20 liter glass or polypropylene bottles.
How do you isolate cyanobacteria from water?
To isolate cyanobacteria from lakes and ponds in which cyanobacterial growth is not visible with the eye (or even after examination with a portable microscope) it is advisable to take larger samples: 250- to 500-ml sterile screw-cap centrifuge pots, filled almost completely with sampling water, are convenient …
What are cyanobacteria Class 11?
– Cyanobacteria are Gram-negative photosynthetic prokaryotes, being the most primitive organisms to have oxygenic photosynthesis. They added oxygen to the atmosphere, which is indispensable for the existence of aerobic forms of living organisms.
Does cyanobacteria go away on its own?
As long as you won’t do any other changes, the Cyanobacteria usually goes away within 2-4 weeks. This is in cases where the nutrients have dropped to very low levels.
What is bold basal medium?
Description: Bold’s Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965).