Why is TCEP used?

Why is TCEP used?

Applications. TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis. It also does not reduce metals used in immobilized metal affinity chromatography. TCEP is particularly useful when labeling cysteine residues with maleimides.

How does TCEP work?

TCEP selectively and completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range. TCEP effectively reduces disulfide bonds over a broad pH range. TCEP is resistant to air oxidation. Compared to DTT, TCEP is more stable, more effective, and able to reduce disulfide bonds at lower pHs.

Is TCEP a stronger reducing agent than DTT?

TCEP HCl has the advantages of being odorless, more powerful, irreversible, more hydrophilic, and more resistant to oxidation in air. DTT is a protective agent for reducing S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes.

Is TCEP better than DTT?

For long-term storage of proteins, TCEP is significantly more stable than DTT without metal chelates such as EGTA in the buffer, whereas DTT is more stable if metal chelates are present. Thus TCEP has advantages over DTT, although the choice of reductant is application specific.

What does TCEP stand for?

TCEP

Acronym	Definition
TCEP	Topanga Coalition for Emergency Preparedness
TCEP	Table de Concertation en Environnement de Portneuf (Canada)
TCEP	Tianjin Capital Environmental Protection
TCEP	Training and Competency Evaluation Program (nursing)

How do you make a TCEP solution?

Add 35 ml of cold molecular biology grade water to the vial, and dissolve the TCEP. This resulting solution is very acidic, with an approximate pH of 2.5. Bring the solution to pH 7.0 with 10 N NaOH or 10 N KOH. Bring the resulting solution to 40 ml with molecular biology grade water.

What is the difference between TCEP and DTT?

What is tctcep reducing resin?

TCEP Reducing Resin. TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reducing proteins over a wider range…

How does TCEP reduce disulfides?

TCEP selectively and completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range. TCEP effectively reduces disulfide bonds over a broad pH range. A pH range (1.5 <9.0) is recommended for the reduction reaction. Reductions frequently require less than 5 minutes at room temperature unlike most other reducing agents.

What is TCEP and how does it work?

Biosynthesis, a provider of reliable and innovative products and services to the life-Sciences, Diagnostic and Pharmaceutical Community. TCEP effectively reduces disulfide bonds over a broad pH range. A pH range (1.5<9.0) is recommended for the reduction reaction.

What is the difference between TCEP and DTT reactions?

TCEP reaction is irreversible, whereas DTT reaction is reversible. Compared to DTT, TCEP is preferred for labeling cysteine residues with maleimides. DTT contains thiols and shows reactivity with maleimides, which reduces the labeling efficiency. You can use either TCEP or DTT to label with iodoacetamide.