Does Bafilomycin A1 inhibit autophagy?

Does Bafilomycin A1 inhibit autophagy?

Bafilomycin A1 is found to inhibit autophagy while induces apoptosis. Bafilomycin A1 is a toxic macrolide antibiotic derived from Streptomyces griseus. Bafilomycin A1 inhibits the short circuit current induced by the outer mantle epithelium (OME).

What is the IC50 of Bafilomycin A1?

The IC50 and maximum inhibition dose of Bafilomycin A1 are 0.17 μM and 0.5 μM, respectively. In addition, Bilomycin A1 inhibits the acid influx with an IC50 value of 0.4 nM. Bafilomycin A1 inhibits the acidification dose-dependently resulting in a lower quenching, and thus a higher fluorescence.

What is the incubation time for Bafilomycin A1?

This medium (1.0 mL), with or without the addition of the V-type ATPase inhibitor bafilomycin A1, is incubated with the filtered homogenate (0.1 mL) for 60 minutes at 23–25 °C. The reaction is stopped by the addition of 1 mL of TCA 3%.

What is the role of Bafilomycin A1 in HeLa cells?

Bafilomycin not only dissipates the low endosomal pH but also blocks transport from early to late endosomes in HeLa cells. Bafilomycin A1 at doses of 0.1-1 μM completely inhibits the acidification of lysosomes revealed by the incubation with acridine orange in BNL CL.2 and A431 cells.