How do you write an unknown bacteria report?
The pronouns “I”, “We” and “They” are not typically used.. For example, instead of writing “I used a TSA agar plate to isolate my unknown,” it is customary to write, “A trypticase soy agar (TSA) plate was used to isolate the unknown.” It is also customary to write in the past tense for most of the report.
How do you write a microbiology lab report?
The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references.
How can you identify an unknown bacterial culture?
Various steps involved in the identification of unknown bacteria are:
- Isolation:
- Staining Reactions:
- Biochemical reactions:
- Indole test:
- Methyl Red Test:
- Voges Proskauer Test:
- Citrate Utilization Test:
- TSI:
What is the purpose of an unknown lab report?
The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of …
How do laboratories identify unknown bacteria?
Identification of unknown bacteria is an integral module in most introductory Microbiology laboratory courses. This laboratory activity typically involves identifying bacteria based on Gram staining for morphology and gram reaction followed by studying their biochemical characteristics.
How do biochemical tests identify unknown bacteria?
Identifying bacteria via analyzing their enzymatic profile Each species of bacteria has specific metabolic needs and relies on different enzymes to fuel those unique needs. The presence of catalase, gelatinase, oxidase, urease, for example, can be used to identify the species of bacteria.
Why do we identify unknown bacteria?
Microbiologists must identify bacterial isolates for several practical reasons: • Medical diagnostics — identifying a pathogen isolated from a patient. Food industry — identifying a microbial contaminant responsible for food spoilage. Research setting — identifying a new isolate which carries out an impor tant process.
What is the final step in a lab report?
Conclusion. Your conclusion should be the final section of your lab report.
How do you find the unknowns in a bacterial culture?
The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate was used. The streak method was used to spread the bacteria across the nutrient agar in hopes of isolating a pure culture of one of the bacteria.
How do you identify bacteria on MacConkey agar?
Blood Agar (BA) to determine the hemolytic properties of both unknown bacteria MacConkey Agar (MAC) that will select for the gram-negative bacterium and also determine whether or not it is a lactose fermenter. Colonies produced on this plate will also be used for biochemical testing to determine its identification.
What is the unknown bacteria on the red agar plate?
The unknown is gram (-) bacteria. The Red agar plate becomes yellow color. The following bacteria are negative to mannitol; Bacillus cereus, Bacillus Subtilis, ,Staphylococcus Epidermidis. The Staphylococcus aureus and Enterococcus faecalis are positive to mannitol. No Clearing on the side of the streak. No color change
What tests were performed on the Gram positive and Gram negative bacteria?
The following tests were performed on the Gram Negative bacteria: The Following Tests were performed on the Gram Positive bacteria: See Table 2 for results. The first test performed on the gram negative bacteria, was a Casein Test.